Ng12p is a Ccr4p-like RNA nuclease essential for the final step in 3'-end processing of 5.8S rRNA in Saccharomyces cerevisiae

被引:44
作者
Faber, AW [1 ]
Van Dijk, M [1 ]
Raué, HA [1 ]
Vos, JC [1 ]
机构
[1] Vrije Univ Amsterdam, Bioctr, Inst Mol Biol Wetenschappen, Dept Biochem & Mol Biol, NL-1081 HV Amsterdam, Netherlands
关键词
D O I
10.1017/S1355838202021027
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Saccharomyces cerevisiae contains three nonessential genes (NGL1, NGL2, and NGL3) that encode proteins containing a domain with similarity to a Mg2+-dependent endonuclease motif present in the mRNA deadenylase Ccr4p. We have investigated a possible role of these proteins in rRNA processing, because for many of the pre-rRNA processing steps, the identity of the responsible nuclease remains elusive. Analysis of RNA isolated from cells in which the NGL2 gene has been inactivated (ngl2Delta) demonstrates that correct 3'-end formation of 5.8S rRNA at site E is strictly dependent on Ngl2p. No role in pre-rRNA processing could be assigned to Ngl1p and Ngl3p. The 3'-extended 5.8S rRNA formed in the ngl2Delta mutant is slightly shorter than the 6S precursor previously shown to accumulate upon combined deletion of the 3' --> 5' exonuclease-encoding REX1 and REX2 genes or upon depletion of the exosomal subunits Rrp40p or Rrp45p. Thus, our data add a further component to the set of nucleases required for correct 3'-end formation of yeast 5.8S rRNA.
引用
收藏
页码:1095 / 1101
页数:7
相关论文
共 30 条
[1]   RNase III cleaves eukaryotic preribosomal RNA at a U3 snoRNP-dependent site [J].
AbouElela, S ;
Igel, H ;
Ares, M .
CELL, 1996, 85 (01) :115-124
[2]   Functions of the exosome in rRNA, snoRNA and snRNA synthesis [J].
Allmang, C ;
Kufel, J ;
Chanfreau, G ;
Mitchell, P ;
Petfalski, E ;
Tollervey, D .
EMBO JOURNAL, 1999, 18 (19) :5399-5410
[3]   Rrp6p, the yeast homologue of the human PM-Scl 100-kDa autoantigen, is essential for efficient 5.8 S rRNA 3′ end formation [J].
Briggs, MW ;
Burkard, KTD ;
Butler, JS .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (21) :13255-13263
[4]   A nuclear 3′-5′ exonuclease involved in mRNA degradation interacts with poly(A) polymerase and the hnRNA protein Npl3p [J].
Burkard, KTD ;
Butler, JS .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (02) :604-616
[5]   CCR4, a 3′-5′ poly(A) RNA and ssDNA exonuclease, is the catalytic component of the cytoplasmic deadenylase [J].
Chen, JJ ;
Chiang, YC ;
Denis, CL .
EMBO JOURNAL, 2002, 21 (06) :1414-1426
[6]   THE RNA OF RNASE MRP IS REQUIRED FOR NORMAL PROCESSING OF RIBOSOMAL-RNA [J].
CHU, S ;
ARCHER, RH ;
ZENGEL, JM ;
LINDAHL, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (02) :659-663
[7]   Role of the ITS2-proximal stem and evidence for indirect recognition of processing sites in pre-rRNA processing in yeast [J].
Côté, CA ;
Peculis, BA .
NUCLEIC ACIDS RESEARCH, 2001, 29 (10) :2106-2116
[8]   Functionally unrelated signalling proteins contain a fold similar to Mg2+-dependent endonucleases [J].
Dlakic, M .
TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (06) :272-273
[9]   Identification of four families of yCCR4- and Mg2+ -dependent endonuclease-related proteins in higher eukaryotes, and characterization of orthologs of yCCR4 with a conserved leucine-rich repeat essential for hCAFI/hPOP2 binding [J].
Dupressoir A. ;
Morel A.-P. ;
Barbot W. ;
Loireau M.-P. ;
Corbo L. ;
Heidmann T. .
BMC Genomics, 2 (1)
[10]   The final step in the formation of 25S rRNA in Saccharomyces cerevisiae is performed by 5′→3′ exonucleases [J].
Geerlings, TH ;
Vos, JC ;
Raué, HA .
RNA, 2000, 6 (12) :1698-1703