Consistent and reproducible slice selection in rodent brain using a novel stereotaxic device for MRI

被引:22
作者
Fricke, ST
Vink, R
Chiodo, C
Cernak, I
Ileva, L
Faden, AI
机构
[1] Georgetown Univ, Dept Neurosci, Washington, DC 20057 USA
[2] Univ Adelaide, Dept Pathol, Adelaide, SA, Australia
[3] ASI Instruments Inc, Warren, MI USA
关键词
stereotaxic; rat; mouse; magnetic resonance spectroscopy; CT; neurotrauma; stroke; PET;
D O I
10.1016/j.jneumeth.2004.01.003
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Typically small animal radiological images are obtained after placing the animal in the center of the imaging device using beds or platforms, and then adjusting the position after obtaining a scout image. Such a process does not permit the reproducible visualization of the same anatomical plane with repeated examinations. We have developed a device that allows stereotaxic placement of an animal in precisely the same position for repeated examinations. The instrument incorporates a full range of physiological monitoring and life support systems including temperature control, anesthesia delivery and respiratory monitoring. Using magnetic resonance imaging (MRI), the accuracy and reliability of this device is demonstrated in a rat traumatic brain injury (TBI) model. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:99 / 102
页数:4
相关论文
共 8 条
[1]   Novel diketopiperazine enhances motor and cognitive recovery after traumatic brain injury in rats and shows neuroprotection in vitro and in vivo [J].
Faden, AI ;
Knoblach, SM ;
Cernak, I ;
Fan, L ;
Vink, R ;
Araldi, GL ;
Fricke, ST ;
Roth, BL ;
Kozikowski, AP .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2003, 23 (03) :342-354
[2]   ENHANCED MAGNETIC-RESONANCE-IMAGING OF THE RAT-BRAIN USING A STEREOTAXIC DEVICE WITH A SMALL HEAD COIL - TECHNICAL NOTE [J].
KAMIRYO, T ;
BERR, SS ;
LEE, KS ;
KASSELL, NF ;
STEINER, L .
ACTA NEUROCHIRURGICA, 1995, 133 (1-2) :87-92
[3]   A SYSTEM FOR STEREOTAXIC IRRADIATION AND MAGNETIC-RESONANCE EVALUATIONS IN THE RAT-BRAIN [J].
KARGER, CP ;
HARTMANN, GH ;
HOFFMANN, U ;
LORENZ, WJ .
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1995, 33 (02) :485-492
[4]   Stereotaxic assembly and procedures for simultaneous electrophysiological and MRI study of conscious rat [J].
Khubchandani, M ;
Mallick, HN ;
Jagannathan, NR ;
Kumar, VM .
MAGNETIC RESONANCE IN MEDICINE, 2003, 49 (05) :962-967
[5]   Imaging brain activity in conscious animals using functional MRI [J].
Lahti, KM ;
Ferris, CF ;
Li, FH ;
Sotak, CH ;
King, JA .
JOURNAL OF NEUROSCIENCE METHODS, 1998, 82 (01) :75-83
[6]   TRAUMATIC BRAIN INJURY IN THE RAT - CHARACTERIZATION OF A LATERAL FLUID-PERCUSSION MODEL [J].
MCINTOSH, TK ;
VINK, R ;
NOBLE, L ;
YAMAKAMI, I ;
FERNYAK, S ;
SOARES, H ;
FADEN, AL .
NEUROSCIENCE, 1989, 28 (01) :233-244
[7]   Volumetric MRI measurements of the tree shrew hippocampus [J].
Ohl, F ;
Michaelis, T ;
Fujimori, H ;
Frahm, J ;
Rensing, S ;
Fuchs, E .
JOURNAL OF NEUROSCIENCE METHODS, 1999, 88 (02) :189-193
[8]  
Wolf OT, 2002, BRAIN RES PROTOC, V10, P41