Amperometric immunosensor for probing complement III (C3) based on immobilizing C3 antibody to a nano-Au monolayer supported by sol-gel-derived carbon ceramic electrode

被引:29
作者
Lei, CX
Yang, Y
Wang, H
Shen, GL
Yu, RQ [1 ]
机构
[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China
[2] Hunan City Univ, Dept Environm Chem & Engn, Yiyang 413049, Peoples R China
基金
中国国家自然科学基金;
关键词
amperometric immunosensor; nano-Au monolayer; carbon ceramic electrode; complement III;
D O I
10.1016/j.aca.2004.01.029
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An electrochemical immunosensor based on nano-size particulate gold (nano-Au) monolayer as sensing interface has been developed for probing complement III (C-3). The thiol functional group-derived carbon ceramic electrode (CCE) was firstly constructed using (3-mercapto-propyl) trimethoxy silane (MPTMOS) as sol-gel monomer. The stable nano-Au monolayer was obtained resulting from covalent combination between nano-Au and thiol group on the surface of CCE. The nano-Au monolayer formed was utilized as a sensing platform for the immobilization Of C-3 antibody (anti-C-3) and subsequent immunoreaction. A competitive immunoassay format was adopted with horseradish peroxidase (HRP)-C-3 as a tracer, hydroquinone and hydrogen peroxide as the enzymatic substrates. The dynamic concentration range for C-3 is 0.08-5.6 mug ml(-1). The feasibility of regenerating nano-Au monolayer for consecutive assays was demonstrated by a simple chemical treatment after each determination. The high stability of formed nano-Au monolayer, readily adsorptive immobilization of antibody on nano-Au monolayer, efficient activity retention of loading immunoreactants as well as the simple operation for the formation of nano-Au monolayer make proposed methodology an attractive alternative for the designing new-type immunosensors. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:379 / 384
页数:6
相关论文
共 28 条
[1]   Gold bands as a suitable surface for enzyme immunoassays [J].
Abad-Villar, EM ;
Fernández-Abedul, MT ;
Costa-García, A .
BIOSENSORS & BIOELECTRONICS, 2002, 17 (09) :797-802
[2]   An indirect perfluorosulfonated ionomer-coated electrochemical immunosensor for the detection of the protein human chorionic gonadotrophin [J].
Chetcuti, AF ;
Wong, DKY ;
Stuart, MC .
ANALYTICAL CHEMISTRY, 1999, 71 (18) :4088-4094
[3]   An electrochemical metalloimmunoassay based on a colloidal gold label [J].
Dequaire, M ;
Degrand, C ;
Limoges, B .
ANALYTICAL CHEMISTRY, 2000, 72 (22) :5521-5528
[4]   SEPARATION-FREE SANDWICH ENZYME IMMUNOASSAYS USING MICROPOROUS GOLD ELECTRODES AND SELF-ASSEMBLED MONOLAYER IMMOBILIZED CAPTURE ANTIBODIES [J].
DUAN, CM ;
MEYERHOFF, ME .
ANALYTICAL CHEMISTRY, 1994, 66 (09) :1369-1377
[5]   Disposable amperometric immunosensor for the detection of polycyclic aromatic hydrocarbons (PAHs) using screen-printed electrodes [J].
Fähnrich, KA ;
Pravda, M ;
Guilbault, GG .
BIOSENSORS & BIOELECTRONICS, 2003, 18 (01) :73-82
[6]   Competitive enzyme immunosensor developed on a renewable carbon paste electrode support [J].
Fernández-Sánchez, C ;
Costa-García, A .
ANALYTICA CHIMICA ACTA, 1999, 402 (1-2) :119-127
[7]   PREPARATION AND CHARACTERIZATION OF AU COLLOID MONOLAYERS [J].
GRABAR, KC ;
FREEMAN, RG ;
HOMMER, MB ;
NATAN, MJ .
ANALYTICAL CHEMISTRY, 1995, 67 (04) :735-743
[8]   VOLTAMMETRIC STUDIES OF COMPOSITE CERAMIC CARBON WORKING ELECTRODES [J].
GUN, G ;
TSIONSKY, M ;
LEV, O .
ANALYTICA CHIMICA ACTA, 1994, 294 (03) :261-270
[9]  
Hernández-Santos D, 2002, ELECTROANAL, V14, P1225, DOI 10.1002/1521-4109(200210)14:18<1225::AID-ELAN1225>3.0.CO
[10]  
2-Z