Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell.: Lines and a trophoblast/choriocarcinoma cell hybrid

被引：33

作者：

Frank, HG

Gunawan, B

Ebeling-Stark, I

Schulten, HJ

Funayama, H

Cremer, U

Huppertz, B

Gaus, G

Kaufmann, P

Füzesi, L

机构：

[1] Aachen Tech Univ, Dept Anat, D-52057 Aachen, Germany

[2] Aachen Tech Univ, Dept Forens Med, D-52057 Aachen, Germany

[3] Univ Gottingen, Dept Pathol, D-3400 Gottingen, Germany

来源：

CANCER GENETICS AND CYTOGENETICS | 2000年 / 116卷 / 01期

关键词：

D O I：

10.1016/S0165-4608(99)00107-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We report the successful fusion of human choriocarcinoma cells with normal human trophoblast cells to a choriocarcinoma/trophoblast hybrid. The hybrid cells ACH1P were derived from fusion of primary male trophoblast cells with the HGPRT-defective choriocarcinoma cell line ACI-I. The karyotypes of the parental choriocarcinoma cell line JEG-3, its HGPRT-defective mutant clones AC1-1, ACZ-5, and AC1-9, and the choriocarcinoma/trophoblast hybrid ACH1P are presented, together with a detailed characterization of the AC1-specific chromosomal marker add(X)(q26) using conventional cytogenetic banding techniques and multiplex-fluorescence in situ hybridization (M-FISH). To our knowledge, this is the first report of a stably proliferating human cell hybrid of trophoblastic origin, providing a unique cell culture model to study trophoblast-related invasion and ifs underlying genetic mechanisms. (C) Elsevier Science Inc., 1999. All rights reserved.

引用

页码：16 / 22

页数：7

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