Production of formyl-CoA during peroxisomal alpha-oxidation of 3-methyl-branched fatty acids

被引：45

作者：

Croes, K ^{[1
]}

VanVeldhoven, PP ^{[1
]}

Mannaerts, GP ^{[1
]}

Casteels, M ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,AFDELING FARMACOL,B-3000 LOUVAIN,BELGIUM

来源：

FEBS LETTERS | 1997年 / 407卷 / 02期

关键词：

hydrolase; phytanic acid; Refsum's disease;

D O I：

10.1016/S0014-5793(97)00343-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Oxidation of 3-methyl-substituted fatty acids was studied in purified rat liver peroxisomes. The experiments revealed that formyl-CoA is formed during the alpha-oxidation process, The amount of formyl-CoA found constituted 2-5% of the amount of formate formed. Under the conditions used, no activation of exogenously added formate occurred in purified peroxisomes, whereas 95.5% of added synthetic formyl-CoA was converted to formate, These data indicate that during alpha-oxidation first formyl-CoA is formed, which is then hydrolysed to formate. (C) 1997 Federation of European Biochemical Societies.

引用

页码：197 / 200

页数：4

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