Aspects in lipase screening

被引：6

作者：

Clausen, IG

机构：

[1] Novo Nordisk A/S, Microbial Discovery Team 1, Building 1B1.18, DK-2880 Bagsvaerd, Novo Alle

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 1997年 / 3卷 / 1-4期

关键词：

lipases; screening; industrial use;

D O I：

10.1016/S1381-1177(96)00037-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipases have become increasingly important for the industry over the last 10 years, and heterologous gene expression has played a key role. Screening of the biological diversity that Nature provides in order to identify lipases suitable for industrial use is a key issue. This paper will discuss some of the aspects to consider when carrying out a lipase screening program.

引用

收藏

页码：139 / 146

页数：8

相关论文

共 13 条

[1] THE FUNGAL DIMENSION OF BIODIVERSITY - MAGNITUDE, SIGNIFICANCE, AND CONSERVATION [J].

HAWKSWORTH, DL .

MYCOLOGICAL RESEARCH, 1991, 95 :641-655

[2] NEW FAMILIES IN THE CLASSIFICATION OF GLYCOSYL HYDROLASES BASED ON AMINO-ACID-SEQUENCE SIMILARITIES [J].

HENRISSAT, B ;

BAIROCH, A .

BIOCHEMICAL JOURNAL, 1993, 293 :781-788

[3] CUTINASE, A LIPOLYTIC ENZYME WITH A PREFORMED OXYANION HOLE [J].

MARTINEZ, C ;

NICOLAS, A ;

VANTILBEURGH, H ;

EGLOFF, MP ;

CUDREY, C ;

VERGER, R ;

CAMBILLAU, C .

BIOCHEMISTRY, 1994, 33 (01) :83-89

[4]

MUNOZ CI, UNPUB ISOLATION CHAR

[5] GENOME STRUCTURE AND NUCLEOTIDE-SEQUENCE OF A LIPOLYTIC ENZYME GENE OF ASPERGILLUS-ORYZAE [J].

OHNISHI, K ;

TOIDA, J ;

NAKAZAWA, H ;

SEKIGUCHI, J .

FEMS MICROBIOLOGY LETTERS, 1995, 126 (02) :145-150

[6] THE ALPHA/BETA-HYDROLASE FOLD [J].

OLLIS, DL ;

CHEAH, E ;

CYGLER, M ;

DIJKSTRA, B ;

FROLOW, F ;

FRANKEN, SM ;

HAREL, M ;

REMINGTON, SJ ;

SILMAN, I ;

SCHRAG, J ;

SUSSMAN, JL ;

VERSCHUEREN, KHG ;

GOLDMAN, A .

PROTEIN ENGINEERING, 1992, 5 (03) :197-211

[7] THE USE OF CONSERVED CELLULASE FAMILY-SPECIFIC SEQUENCES TO CLONE CELLULASE HOMOLOG CDNAS FROM FUSARIUM-OXYSPORUM [J].

SHEPPARD, PO ;

GRANT, FJ ;

OORT, PJ ;

SPRECHER, CA ;

FOSTER, DC ;

HAGEN, FS ;

UPSHALL, A ;

MCKNIGHT, GL ;

OHARA, PJ .

GENE, 1994, 150 (01) :163-167

[8] STRUCTURE OF THE CUTINASE GENE AND DETECTION OF PROMOTER ACTIVITY IN THE 5'-FLANKING REGION BY FUNGAL TRANSFORMATION [J].

SOLIDAY, CL ;

DICKMAN, MB ;

KOLATTUKUDY, PE .

JOURNAL OF BACTERIOLOGY, 1989, 171 (04) :1942-1951

[9] COMPARISON OF NUCLEIC-ACIDS FROM MICROORGANISMS - SEQUENCING APPROACHES [J].

STAHL, D .

MOLECULAR EVOLUTION: PRODUCING THE BIOCHEMICAL DATA, 1993, 224 :373-391

[10] CLONING AND ANALYSIS OF CUT1, A CUTINASE GENE FROM MAGNAPORTHE-GRISEA [J].

SWEIGARD, JA ;

CHUMLEY, FG ;

VALENT, B .

MOLECULAR & GENERAL GENETICS, 1992, 232 (02) :174-182