Urinary nitrate excretion in cholesterol-fed rabbits:: effect of a chronic treatment by N-iminoethyl-L-lysine, a selective inhibitor of inducible nitric oxide synthase

被引:7
作者
Behr-Roussel, D
Rupin, A
Simonet, S
Fabiani, JN
Verbeuren, TJ
机构
[1] Inst Rech Servier, Div Angiol, F-92150 Suresnes, France
[2] Hop Broussais, Dept Cardiovasc Surg, F-75014 Paris, France
[3] Hop Broussais, Lab Etud Greffes & Protheses Cardiaques, F-75014 Paris, France
关键词
(rabbit); nitrate; atherosclerosis; nitric oxide (NO) synthase; nitric oxide (NO); L-NIL (N-iminoethyl-L-lysine);
D O I
10.1016/S0014-2999(99)00836-5
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
To evaluate the influence of atherosclerosis on the global production of NO, we studied the effect of a 0.3% cholesterol-enriched diet on urinary nitrate excretion in rabbits during 69 weeks. To examine whether the inducible nitric oxide synthase (iNOS) present in atherosclerotic lesions could participate in NO excretion, hypercholesterolemic rabbits were treated chronically with the selective iNOS inhibitor, N-iminoethyl-L-lysine (L-NIL; 5 mg/kg/day). Urinary nitrate excretion was higher in hypercholesterolemic than in control rabbits throughout the study period and decreased progressively with time in both groups; L-NIL had no significant effect on urinary nitrate excretion. These data illustrate that systemic NO production is enhanced in hypercholesterolemia and that iNOS, present within the plaque, might not participate in this enhanced NO production. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:275 / 279
页数:5
相关论文
共 23 条
[1]   Measurement of nitrite and nitrate levels in plasma and urine - what does this measure tell us about the activity of the endogenous nitric oxide system? [J].
Baylis, C ;
Vallance, P .
CURRENT OPINION IN NEPHROLOGY AND HYPERTENSION, 1998, 7 (01) :59-62
[2]   Distribution and prevalence of inducible nitric oxide synthase in atherosclerotic vessels of long-term cholesterol-fed rabbits [J].
Behr, D ;
Rupin, A ;
Fabiani, JN ;
Verbeuren, TJ .
ATHEROSCLEROSIS, 1999, 142 (02) :335-344
[3]  
BOGER RH, 1995, ATHEROSCLEROSIS, V117, P273, DOI 10.1016/0021-9150(95)05582-H
[4]   Dietary L-arginine reduces the progression of atherosclerosis in cholesterol-fed rabbits - Comparison with lovastatin [J].
Boger, RH ;
BodeBoger, SM ;
Brandes, RP ;
Phivthongngam, L ;
Bohme, M ;
Nafe, R ;
Mugge, A ;
Frolich, JC .
CIRCULATION, 1997, 96 (04) :1282-1290
[5]   Expression of inducible nitric oxide synthase in T lymphocytes and macrophages of cholesterol-fed rabbits [J].
Esaki, T ;
Hayashi, T ;
Muto, E ;
Yamada, K ;
Kuzuya, M ;
Iguchi, A .
ATHEROSCLEROSIS, 1997, 128 (01) :39-46
[6]   2-amino-4-methylpyridine as a potent inhibitor of inducible NO synthase activity in vitro and in vivo [J].
Faraci, WS ;
Nagel, AA ;
Verdries, KA ;
Vincent, LA ;
Xu, H ;
Nichols, LE ;
Labasi, JM ;
Salter, ED ;
Pettipher, ER .
BRITISH JOURNAL OF PHARMACOLOGY, 1996, 119 (06) :1101-1108
[7]   1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo [J].
Garvey, EP ;
Oplinger, JA ;
Furfine, ES ;
Kiff, RJ ;
Laszlo, F ;
Whittle, BJR ;
Knowles, RG .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (08) :4959-4963
[8]  
GRANGER DL, 1991, J IMMUNOL, V146, P1294
[9]   Acetamidine lysine derivative, N-(5(S)-amino-6,7-dihydroxyheptyl)-ethanimidamide dihydrochloride:: A highly selective inhibitor of human inducible nitric oxide synthase [J].
Hallinan, EA ;
Tsymbalov, S ;
Finnegan, PM ;
Moore, WM ;
Jerome, GM ;
Currie, MG ;
Pitzele, BS .
JOURNAL OF MEDICINAL CHEMISTRY, 1998, 41 (06) :775-777
[10]   OXIDATION OF NITRIC-OXIDE IN AQUEOUS-SOLUTION TO NITRITE BUT NOT NITRATE - COMPARISON WITH ENZYMATICALLY FORMED NITRIC-OXIDE FROM L-ARGININE [J].
IGNARRO, LJ ;
FUKUTO, JM ;
GRISCAVAGE, JM ;
ROGERS, NE ;
BYRNS, RE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8103-8107