Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment

In this work, in vitro biosynthesis of silver nanoparticles was achieved using AgNO3 as a substrate by Penicillium fellutanum isolated from coastal mangrove sediment. The biosynthesis was faster within minutes of silver ion coming in contact with the cell filtrate. Presence of silver nanoparticles in the culture filtrate was confirmed by absorption peak at 430 nm, as well under transmission electron microscope. The biosynthesis of nanoparticles was the maximum when the culture filtrate was treated with 1.0 mM AgNO3, maintained at 0.3% NaCl and pH 6.0, incubated at 5 degrees C for 24 h. The culture filtrate. precipitated with ammonium sulphate, was proved to have a single protein band with a molecular weight of 70 kDa using polyacrylamide gel electrophoresis. The present work highlighted the possibility of using the marine fungal strain of P.fellutanum to achieve a fast rate of nanoparticles synthesis. (C) 2009 Elsevier B.V. All rights reserved.