Induced expression of hematopoietic- and neurologic-expressed sequence 1 in retinal pigment epithelial cells during newt retina regeneration

被引：21

作者：

Goto, Tatsushi ^{[1
]}

Hisatomi, Osamu ^{[1
]}

Kotoura, Mari ^{[1
]}

Tokunaga, Fumio ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Sci, Osaka 5600043, Japan

来源：

EXPERIMENTAL EYE RESEARCH | 2006年 / 83卷 / 04期

关键词：

retina regeneration; progenitor cell; differential display; Hn1; retinal pigment epithelium;

D O I：

10.1016/j.exer.2006.05.004

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Newts can regenerate their organs even as adults. For instance, when their neural retinas are completely removed by operation, the remaining retinal pigment epithelial (RPE) cells dedifferentiate to reconstruct neural retinas. To elucidate the molecular mechanisms of newt retina regeneration, we investigated genes upregulated in dedifferentiating RPE cells using differential display methods. We observed that a cDNA fragment of hematopoietic- and neurologic-expressed sequence I (Hn1) appeared to be induced within a few days of surgical removal of newt neural retina. Using an anti-HN1 antiserum against the recombinant HN1 protein, we carried out immunohistochemical analyses. The anti-HN1 antiserum recognized the plexiform layers and ganglion cell layer (GCL) but not the RPE cell layer in unoperated (normal) newt retinas. Using a glial fibrillary acidic protein antibody, Hut was shown to be possibly expressed in glial cells in normal neural retina. During retina regeneration, immunoreactivity for HN1 appeared in dedifferentiating RPE cells 10 days post-operation, and in retinal progenitor cells 18 days postoperation. Twenty seven days post-operation, HN1 immunoreactivity was localized in the plexiform layers and GCL as in the normal retina. Therefore, HN1 possibly plays an undefined role in dedifferentiating RPE cells and retinal progenitor cells during newt retina regeneration. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：972 / 980

页数：9

共 29 条

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