A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA

被引：58

作者：

Jackson, D

Cadman, A

Zurcher, T

Barclay, WS

机构：

[1] Univ Reading, Sch Anim & Microbial Sci, Reading RG6 2AJ, Berks, England

[2] GlaxoSmithKline Med Res Ctr, Stevenage SG1 2NY, Herts, England

来源：

JOURNAL OF VIROLOGY | 2002年 / 76卷 / 22期

关键词：

D O I：

10.1128/JVI.76.22.11744-11747.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.

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收藏

页码：11744 / 11747

页数：4

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