CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis

被引：68

作者：

Tamura, Keita ^{[1
]}

Seki, Hikaru ^{[1
]}

Suzuki, Hideyuki ^{[2
]}

Kojoma, Mareshige ^{[3
]}

Saito, Kazuki ^{[4
]}

Muranaka, Toshiya ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Dept Biotechnol, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan

[2] Kazusa DNA Res Inst, Dept Res & Dev, 2-6-7 Kazusa Kamatari, Kisarazu, Chiba 2920818, Japan

[3] Hlth Sci Univ Hokkaido, Fac Pharmaceut Sci, 1757 Kanazawa, Tobetsu, Hokkaido 0610293, Japan

[4] Chiba Univ, Grad Sch Pharmaceut Sci, Chuo Ku, 1-8-1 Inohana, Chiba 2608675, Japan

来源：

PLANT CELL REPORTS | 2017年 / 36卷 / 03期

关键词：

Glycyrrhiza uralensis; Licorice; P450; RNA-seq; Triterpenoid; BIOSYNTHESIS; LICORICE; ORGANS;

D O I：

10.1007/s00299-016-2092-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

CYP716A179, a cytochrome P450 monooxygenase expressed predominantly in tissue-cultured stolons of licorice ( Glycyrrhiza uralensis ), functions as a triterpene C-28 oxidase in the biosynthesis of oleanolic acid and betulinic acid. Cytochrome P450 monooxygenases (P450s) play key roles in the structural diversification of plant triterpenoids. Among these, the CYP716A subfamily, which functions mainly as a triterpene C-28 oxidase, is common in plants. Licorice (Glycyrrhiza uralensis) produces bioactive triterpenoids, such as glycyrrhizin and soyasaponins, and relevant P450s (CYP88D6, CYP72A154, and CYP93E3) have been identified; however, no CYP716A subfamily P450 has been isolated. Here, we identify CYP716A179, which functions as a triterpene C-28 oxidase, by RNA sequencing analysis of tissue-cultured stolons of G. uralensis. Heterologous expression of CYP716A179 in engineered yeast strains confirmed the production of oleanolic acid, ursolic acid, and betulinic acid from beta-amyrin, alpha-amyrin, and lupeol, respectively. The transcript level of CYP716A179 was about 500 times higher in tissue-cultured stolons than in intact roots. Oleanolic acid and betulinic acid were consistently detected only in tissue-cultured stolons. The discovery of CYP716A179 helps increase our understanding of the mechanisms of tissue-type-dependent triterpenoid metabolism in licorice and provides an additional target gene for pathway engineering to increase the production of glycyrrhizin in licorice tissue cultures by disrupting competing pathways.

引用

页码：437 / 445

页数：9

共 22 条

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