The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis

被引:780
作者
Teige, M
Scheikl, E
Eulgem, T
Dóczi, F
Ichimura, K
Shinozaki, K
Dangl, JL
Hirt, H
机构
[1] Univ Vienna, Max F Perutz Labs, A-1030 Vienna, Austria
[2] Austrian Acad Sci, Vienna Bioctr, Gregor Mendel Inst Mol Plant Sci, A-1030 Vienna, Austria
[3] Univ N Carolina, Dept Biol, Curriculum Genet, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA
[4] RIKEN, Tsukuba Inst, Plant Mol Biol Lab, Tsukuba, Ibaraki 3050074, Japan
基金
奥地利科学基金会;
关键词
D O I
10.1016/j.molcel.2004.06.023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Arabidopsis mitogen-activated protein kinase (MAPK) kinase 2 (MKK2) and the downstream MAPKs MPK4 and MPK6 were isolated by functional complementation of osmosensitive yeast mutants. In Arabidopsis protoplasts, MKK2 was specifically activated by cold and salt stress and by the stress-induced MAPK kinase kinase MEKK1. Yeast two-hybrid, in vitro, and in vivo protein kinase assays revealed that MKK2 directly targets MPK4 and MPK6. Accordingly, plants overexpressing MKK2 exhibited constitutive MPK4 and MPK6 activity, constitutively upregulated expression of stress-induced marker genes, and increased freezing and salt tolerance. In contrast, mkk2 null plants were impaired in MPK4 and MPK6 activation and were hypersensitive to salt and cold stress. Full genome transcriptome analysis of MKK2-overexpressing plants demonstrated altered expression of 152 genes involved in transcriptional regulation, signal transduction, cellular defense, and stress metabolism. These data identify a MAP kinase signaling cascade mediating cold and salt stress tolerance in plants.
引用
收藏
页码:141 / 152
页数:12
相关论文
共 52 条
[1]   MAP kinase signalling cascade in Arabidopsis innate immunity [J].
Asai, T ;
Tena, G ;
Plotnikova, J ;
Willmann, MR ;
Chiu, WL ;
Gomez-Gomez, L ;
Boller, T ;
Ausubel, FM ;
Sheen, J .
NATURE, 2002, 415 (6875) :977-983
[2]   A MAP kinase is activated late in plant mitosis and becomes localized to the plane of cell division [J].
Bögre, L ;
Calderini, O ;
Binarova, P ;
Mattauch, M ;
Till, S ;
Kiegerl, S ;
Jonak, C ;
Pollaschek, C ;
Barker, P ;
Huskisson, NS ;
Hirt, H ;
Heberle-Bors, E .
PLANT CELL, 1999, 11 (01) :101-113
[3]   Differential activation of four specific MAPK pathways by distinct elicitors [J].
Cardinale, F ;
Jonak, C ;
Ligterink, W ;
Niehaus, K ;
Boller, T ;
Hirt, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (47) :36734-36740
[4]   Role of nicotianamine in the intracellular delivery of metals and plant reproductive development [J].
Takahashi, M ;
Terada, Y ;
Nakai, I ;
Nakanishi, H ;
Yoshimura, E ;
Mori, S ;
Nishizawa, NK .
PLANT CELL, 2003, 15 (06) :1263-1280
[5]   Changes in gene expression during the early to mid-luteal (receptive phase) transition in human endometrium detected by high-density microarray screening [J].
Carson, DD ;
Lagow, E ;
Thathiah, A ;
Al-Shami, R ;
Farach-Carson, MC ;
Vernon, M ;
Yuan, LW ;
Fritz, MA ;
Lessey, B .
MOLECULAR HUMAN REPRODUCTION, 2002, 8 (09) :871-879
[6]   Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis [J].
Cheong, YH ;
Chang, HS ;
Gupta, R ;
Wang, X ;
Zhu, T ;
Luan, S .
PLANT PHYSIOLOGY, 2002, 129 (02) :661-677
[7]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743
[8]   Signal transduction by the JNK group of MAP kinases [J].
Davis, RJ .
CELL, 2000, 103 (02) :239-252
[9]   Harpin induces activation of the arabidopsis mitogen-activated protein kinases AtMPK4 and AtMPK6 [J].
Desikan, R ;
Hancock, JT ;
Ichimura, K ;
Shinozaki, K ;
Neill, SJ .
PLANT PHYSIOLOGY, 2001, 126 (04) :1579-1587
[10]   Different protein kinase families are activated by osmotic stresses in Arabidopsis thaliana cell suspensions -: Involvement of the MAP kinases AtMPK3 and AtMPK6 [J].
Droillard, MJ ;
Boudsocq, M ;
Barbler-Brygoo, H ;
Laurière, C .
FEBS LETTERS, 2002, 527 (1-3) :43-50