Determination of the antioxidant activity of phenolic compounds by coulometric detection

被引：117

作者：

Peyrat-Maillard, MN ^{[1
]}

Bonnely, S ^{[1
]}

Berset, C ^{[1
]}

机构：

[1] ENSIA, Dept Sci Aliment, Lab Chim Subst Nat Antioxydants Aromes Colorants, F-91744 Massy, France

来源：

TALANTA | 2000年 / 51卷 / 04期

关键词：

antioxidant activity; electrochemical detection; phenolic compounds; flavonoids;

D O I：

10.1016/S0039-9140(99)00331-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The combination of reversed-phase high-performance liquid chromatography with coulometric detection allows the detection of phenolic antioxidants in complex matrices like plant extracts with a high degree of sensitivity and selectivity. According to their voltammetric behaviour, phenolic acids and tocopherols show maximal detector response at low potentials (100-450 mV) while flavonoids show optimal response at two different potential values tone at 0-300 mV and one at 600-900 mV). The potential corresponding to maximal detector response (MDRP) of phenolic acids was shown to be inversely proportional to their antioxidant efficiency as determined in a lipidic model system under strong oxidizing conditions (110 degrees C, intensive oxygenation) or by the DPPH. test. However, such a relationship was not observed for flavonoids. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：709 / 716

页数：8

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