E-selectin is upregulated in proliferating endothelial cells In vitro

Objective: E-selectin is an endothelial cell-specific membrane glycoprotein that participates in leukocyte adhesion and has also been suggested to function in angiogenesis. To gain further insights into E-selectin, we analyzed E-selectin polypeptide in proliferating versus quiescent bovine capillary endothelial cells and its expression as a function of the cell cycle. Methods: E-selectin polypeptide was analyzed by immunoadsorption from (35)Scysteine-labeled endothelial cells, by enzyme-linked immunosorbent assay, and by fluorescence-activated cell sorting. The distribution of endothelial cells in G(0)/G(1), S, and G(2)/M phases of the cell cycle was determined using propidium iodide staining of DNA. Results: E-selectin was upregulated in subconfluent proliferating bovine capillar?i endothelial cells compared to confluent quiescent cultures. The upregulation was independent of activation in that E-selectin was further increased by treatment with tumor necrosis factor alpha or lipopolysaccharide. In contrast to E-selectin, P-selectin and platelet-endothelial cell adhesion molecule-1 did not appear to be regulated by the growth state of the endothelial cells. The distribution of E-selectin-positive cells in G(0)G(1), S, and G(2)/M phases of the cell cycle differed from E-selectin-negative cells in that more of the E-selectin-positive cells were in G(2) and M. Conclusions: Increased E-selectin expression under noninflammatory conditions is correlated with cellular proliferation and G(2)/M phases of the cell cycle. The expression of E-selectin in proliferating endothelial cells in vitro is consistent with the presence of E-selectin in proliferating endothelial cells in vivo (Kraling et al. [18]).