Forced expression of essential myosin light chain isoforms demonstrates their role in smooth muscle force production

被引:26
作者
Huang, QQ
Fisher, SA
Brozovich, FV [1 ]
机构
[1] Case Western Reserve Univ, Dept Med Cardiol, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Dept Physiol & Biophys, Cleveland, OH 44106 USA
关键词
D O I
10.1074/jbc.274.49.35095
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The molecular determinants of the contractile properties of smooth muscle are poorly understood, and have been suggested to be controlled by splice variant expression of the myosin heavy chain near the 25/50-kDa junction (Kelley, C. A., Takahashi, M., Yu, J. H., and Adelstein, R. S. (1993) J. Biol. Chern. 268, 12848-12854) as well as by differences in the expression of an acidic (MLC17a) and a basic (MLC17b) isoform of the 17-kDa essential myosin light chain (Nabeshima, Y., Nonomura, Y., and Fujii-Kuriyama, Y. (1987) J. Biol. Chem. 262, 106508-10612). To investigate the molecular mechanism that regulates the mechanical properties of smooth muscle, we determined the effect of forced expression of MLC17a and MLC17b on the rate of force activation during agonist-stimulated contractions of single cultured chicken embryonic aortic and gizzard smooth muscle cells. Forced expression of MLC17a in aortic smooth muscle cells increased (p < 0.05) the rate of force activation, forced expression of MLC17b in gizzard smooth muscle cells decreased (p < 0.05) the rate of force activation, while forced expression of the endogenous MLC17 isoform had no effect on the rate of force activation. These results demonstrate that MLC17 is a molecular determinant of the contractile properties of smooth muscle. MLC17 could affect the contractile properties of smooth muscle by either changing the stiffness of the myosin lever arm or modulating the rate of a load-dependent step and/or transition in the actomyosin ATPase cycle.
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页码:35095 / 35098
页数:4
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