High level expression of the key antigenic protein, σC, from avian reovirus into insect cells and its purification by immobilized metal affinity chromatography

被引：16

作者：

Hu, YC ^{[1
]}

Liu, HJ

Chung, YC

机构：

[1] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu, Taiwan

[2] Natl Pingtung Univ Sci & Technol, Dept Vet Med, Pingtung, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 12期

关键词：

avian reovirus; baculovirus; enterokinase; immobilized metal affinity chromatography; purification;

D O I：

10.1023/A:1015689313560

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Avian reovirus (ARV) structural protein, sigmaC, the prime candidate for vaccine against ARV, was expressed using a baculovirus/insect cell system. The expressed protein remained intracellular and reached 96 mug/10(6) cells. Total product yield from a 200 ml suspension culture was 19 mg. When the protein was fused with a histidine tag and an enterokinase (EK) cleavage site, purification of 94% was achieved in a single step. The histidine tag was removed by EK.

引用

页码：1017 / 1022

页数：6

共 18 条

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2-K