A simple method for 3'-labeling of RNA

被引：75

作者：

Huang, Z ^{[1
]}

Szostak, JW ^{[1
]}

机构：

[1] MASSACHUSETTS GEN HOSP,DEPT MOL BIOL,BOSTON,MA 02114

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 21期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/24.21.4360

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a simple method for 3'-end labeling RNAs of known sequence. A short DNA template is designed to anneal to the 3'-end of the RNA, with a two nucleotide 5' overhang of 3'-TA-5', 3'-TG-5' or 3'-TC-5'. The Klenow fragment of DNA polymerase I can then cleanly and efficiently extend the 3'-end of the RNA by the incorporation of a single alpha-P-32-labeled dATP residue. This method can be used to label one RNA in a mixture of RNAs, or to label 5'-blocked RNAs such as mRNA.

引用

页码：4360 / 4361

页数：2

共 14 条

[1]

BELTZ WR, 1982, FED PROC, V41, P1450

[2]

EDMONDS M, 1982, ENZYMES, V15, P217

[3] 3'-TERMINAL LABELING OF RNA WITH T4 RNA LIGASE [J].