Discovery of transcriptional regulators and signaling pathways in the developing pituitary gland by bioinformatic and genomic approaches

被引：61

作者：

Brinkmeier, Michelle L. ^{[1
]}

Davis, Shannon W. ^{[1
]}

Carninci, Piero ^{[2
]}

MacDonald, James W. ^{[3
]}

Kawai, Jun ^{[2
]}

Ghosh, Debashis ^{[5
]}

Hayashizaki, Yoshihide ^{[2
]}

Lyons, Robert H. ^{[4
]}

Camper, Sally A. ^{[1
]}

机构：

[1] Univ Michigan, Sch Med, Dept Human Genet, Ann Arbor, MI 48109 USA

[2] Yokohama Inst, RIKEN, Om Sci Ctr, Tsurumi Ku, Kanagawa 2300045, Japan

[3] Univ Michigan, Ctr Canc, Affymetrix & cDNA Microarray Core Facil, Ann Arbor, MI 48109 USA

[4] Univ Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Sch Publ Hlth, Dept Biostat, Ann Arbor, MI 48109 USA

来源：

GENOMICS | 2009年 / 93卷 / 05期

基金：

美国国家卫生研究院;

关键词：

Cap trapper; Homeobox gene; Prop1; Gene expression; Ames dwarf; FULL-LENGTH CDNA; GONADOTROPIN-RELEASING-HORMONE; AMES DWARF GENE; ANTERIOR-PITUITARY; EMBRYONIC PITUITARY; CELL-PROLIFERATION; LINEAGE DETERMINATION; VENTRAL DIENCEPHALON; EXPRESSION PATTERNS; LUTEINIZING-HORMONE;

D O I：

10.1016/j.ygeno.2008.11.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report a catalog of the mouse embryonic pituitary gland transcriptome consisting of five cDNA libraries including wild type tissue from E12.5 and E14.5, Prop1(df/df) mutant at E14.5 and two cDNA subtractions: E14.5 WT-E14.5 Prop1(df/df) and E14.5 WT-E12.5 WT. DNA sequence information is assembled into a searchable database with gene ontology terms representing 12,009 expressed genes. We validated coverage of the libraries by detecting most known homeobox gene transcription factor cDNAs. A total of 45 homeobox genes were detected as part of the pituitary transcriptome, representing most expected ones, which validated library coverage, and many novel ones, underscoring the utility of this resource as a discovery tool. We took a similar approach for signaling-pathway members with novel pituitary expression and found 157 genes related to the BMP, FGF, WNT, SHH and NOTCH pathways. These genes are exciting candidates for regulators of pituitary development and function. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：449 / 460

页数：12

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