Oxidative stress in Lake Sturgeon (Acipenser fulvescens) orally exposed to 2,3,7,8-tetrachlorodibenzofuran

被引：42

作者：

Palace, VP ^{[1
]}

Dick, TA ^{[1
]}

Brown, SB ^{[1
]}

Baron, CL ^{[1
]}

Klaverkamp, JF ^{[1
]}

机构：

[1] FISHERIES & OCEANS CANADA,INST FRESHWATER,DEPT FISHERIES & OCEANS,WINNIPEG,MB R3T 2N6,CANADA

来源：

AQUATIC TOXICOLOGY | 1996年 / 35卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

oxidative stress; sturgeon; TCDF; EROD; DIBENZO-PARA-DIOXINS; RAINBOW-TROUT; LIPID-PEROXIDATION; VITAMIN-A; CYTOCHROME-P-450; SYSTEM; EROD ACTIVITY; ASSAY-METHOD; FISH; DISPOSITION; MECHANISMS;

D O I：

10.1016/0166-445X(95)00051-5

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Juvenile lake sturgeon were orally dosed with gelatin containing nominal concentrations of 0, 0.16 or 1.6 ng [H-3]-2,3,7,8-tetrachlorodibenzofuran (TCDF) kg(-1) fish weight. Liver, kidney and blood were collected 10 and 27 days after exposure. Phase I mixed-function oxidase (MFO) and Phase II (glucuronyltransferase) enzyme activities were determined in liver. Concentrations of non-enzymatic (tocopherol, retinoids) and enzymatic (superoxide dismutase, catalase, glutathione peroxidase) antioxidant parameters were also quantified in liver and kidney. TC DF concentrations were elevated in liver and kidney of both dose groups at 10 and 27 days. Lower tissue concentrations of TCDF at 27 days, compared with 10 day exposures, as well as the presence of polar metabolites in bile, may indicate rapid metabolism and clearance of the contaminant. MFO enzyme activity, measured as ethoxyresorufin-O-deethylase (EROD), was induced in liver with activity at the two sample periods reflecting the TCDF concentrations. Greater concentrations of hepatic lipid peroxides in both dose groups, indicate that oxidative stress was produced by contaminant exposure and/or induced metabolism enzyme activity.

引用

页码：79 / 92

页数：14

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