Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis

被引:86
作者
Atkins, T [1 ]
Prior, R
Mack, K
Russell, P
Nelson, M
Prior, J
Ellis, J
Oyston, PCF
Dougan, G
Titball, RW
机构
[1] CBS Porton Down, Def Sci & Technol Lab, Salisbury SP4 0JQ, Wilts, England
[2] Univ London Imperial Coll Sci Technol & Med, Ctr Mol Microbiol & Infect, Dept Biol Sci, London SW7 2AY, England
[3] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Med, London WC1E 7HT, England
关键词
D O I
10.1099/0022-1317-51-7-539
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A Burkholderia pseudomallei mutant which was attenuated in a mouse model of melioidosis was identified by a signature tagged mutagenesis approach. The transposon was shown to be inserted into a gene within the capsular biosynthetic operon. Compared with the wild-type bacteria this mutant demonstrated a 10(5)-fold increase in the median lethal dose in a mouse model and it did not react with a monoclonal antibody against high mol. wt polysaccharide of B. pseudomallei. To determine the kinetics of infection, mice were dosed intraperitoneally (i.p.) and intravenously (i.v.) with, mutant and wildtype bacteria. After i.p challenge, the number of mutant bacteria in the peritoneal cavity declined, whereas wild-type bacteria proliferated. When administered by the i.v. route, the mutant was able to cause disease but the time to death was increased compared with the wild type. Mice were dosed with the mutant and subsequently challenged with wildtype B. pseudomallei, but the mutant failed to induce a protective immune response.
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页码:539 / 547
页数:9
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