Resveratrol increases glutamate uptake and glutamine synthetase activity in C6 glioma cells

被引：115

作者：

dos Santos, Andre Quincozes ^{[1
]}

Nardin, Patricia ^{[1
]}

Funchal, Claudia ^{[1
]}

Vielra de Almeida, Lucia Maria ^{[1
]}

Jacques-Silva, Maria Caroline ^{[1
]}

Wofchuk, Susana T. ^{[1
]}

Goncalves, Carlos-Alberto ^{[1
]}

Gottfried, Carmem ^{[1
]}

机构：

[1] Univ Fed Rio Grande Sul, Inst Ciencias Basicas Saude, Dept Bioquim, BR-90035003 Porto Alegre, RS, Brazil

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2006年 / 453卷 / 02期

关键词：

resveratrol; glutamate uptake; glutamine synthetase; C6; glioma; GFAP; S100B;

D O I：

10.1016/j.abb.2006.06.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Resveratrol, a phytoalexin found mainly in grapes, is a promising natural product with anti-cancer and cardio-protective activities. Here, we investigated, in C6 glioma cells, the effect of resveratrol on some specific parameters of astrocyte activity (glutamate uptake, glutamine synthetase and secretion of S 10013, a neurotrophic cytokine) commonly associated with the protective role of these cells. Cell proliferation was significantly decreased by 8% and 26%, following 24 h of treatment with 100 and 250 mu M resveratrol. Extracellular S100B increased after 48 h of resveratrol exposure. Short-term resveratrol exposure (from 1 to 100 mu M) induced a linear increase in glutamate uptake (up to 50% at 100 mu M resveratrol) and in glutamine synthetase activity. Changes in these glial activities can contribute to the protective role of astrocytes in brain injury conditions, reinforcing the putative use of this compound in the therapeutic arsenal against neurodegenerative diseases and ischemic disorders. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：161 / 167

页数：7

共 55 条

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