Bone mineral content and density in the humerus of adult myostatin-deficient mice

被引:62
作者
Hamrick, MW [1 ]
McPherron, AC
Lovejoy, CO
机构
[1] Med Coll Georgia, Dept Cellular Biol & Anat, Augusta, GA 30912 USA
[2] Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA
[3] Kent State Univ, Dept Anthropol, Kent, OH 44242 USA
[4] Kent State Univ, Sch Biomed Sci, Kent, OH 44242 USA
关键词
GDF-8; muscle mass; pQCT; mechanical stress; cortical bone; trabecular bone;
D O I
10.1007/s00223-001-1109-8
中图分类号
R5 [内科学];
学科分类号
1002 [临床医学]; 100201 [内科学];
摘要
Myostatin (GDF-8), a member of the transforming growth factor-beta superfamily of secreted growth and differentiation factors, is a negative regulator of skeletal muscle growth. We investigated the effects of increased muscle mass on bone morphology by examining bone mineral content and density in the humeri of myostatin-deficient mice. We compared the humeri of I I mixed-gender, adult mice homozygous for the disrupted myostatin sequence with those from I I mixed-gender, adult wild-type mice. Body mass, deltoid mass, and triceps mass were recorded from each animal and densitometric and geometric parameters were collected from the humerus using peripheral quantitative computed tomography (pQCT). Cross-sectional slices were scanned at four different positions along the humerus corresponding to 15%, 40%, 60%, and 85% of total humerus length. Results show that the myostatin-deficient mice weigh more than controls and have significantly larger triceps and deltoid muscles. The myostatin-deficient animals also have significantly (P < 0.05) higher trabecular area and trabecular bone mineral content (BMC) in the proximal humerus (15% length) and significantly (P < 0.01) higher cortical BMC, cortical area, and periosteal circumference in the region of the deltoid crest (40% length). The myostatin knockouts otherwise do not differ from controls in cortical BMC. Moreover, experimental and control mice do not differ significantly from one another in cortical bone mineral density (BMD) at any of the sites examined. These results suggest that the effects of increased muscle mass on the mouse humerus are localized to regions where muscles attach; furthermore, these effects include increased mineral content of both mineral content of both trabecular and cortical bone.
引用
收藏
页码:63 / 68
页数:6
相关论文
共 31 条
[1]
Bone mineral density and muscle strength in young men with mental retardation (With and without Down syndrome) [J].
Angelopoulou, N ;
Matziari, C ;
Tsimaras, V ;
Sakadamis, A ;
Souftas, V ;
Mandroukas, K .
CALCIFIED TISSUE INTERNATIONAL, 2000, 66 (03) :176-180
[2]
Genetic variability in adult bone density among inbred strains of mice [J].
Beamer, WG ;
Donahue, LR ;
Rosen, CJ ;
Baylink, DJ .
BONE, 1996, 18 (05) :397-403
[3]
THE LAW OF BONE TRANSFORMATION - A CASE OF CRYING WOLFF [J].
BERTRAM, JEA ;
SWARTZ, SM .
BIOLOGICAL REVIEWS, 1991, 66 (03) :245-273
[4]
BEVIER WC, 1989, J BONE MINER RES, V4, P421
[5]
CHARACTERISTICS OF GROWTH-INDUCING EXERCISE [J].
BORER, KT .
PHYSIOLOGY & BEHAVIOR, 1980, 24 (04) :713-720
[6]
Muscle strength, bone mass, and age-related bone loss [J].
Burr, DB .
JOURNAL OF BONE AND MINERAL RESEARCH, 1997, 12 (10) :1547-1551
[7]
RELATION BETWEEN BONE MASS AND MUSCLE WEIGHT [J].
DOYLE, F ;
BROWN, J ;
LACHANCE, C .
LANCET, 1970, 1 (7643) :391-&
[8]
EXERCISE AND ITS INTERACTION WITH GENETIC INFLUENCES IN THE DETERMINATION OF BONE-MINERAL DENSITY [J].
EISMAN, JA ;
SAMBROOK, PN ;
KELLY, PJ ;
POCOCK, NA .
AMERICAN JOURNAL OF MEDICINE, 1991, 91 :S5-S9
[9]
PERSPECTIVES OF PQCT TECHNOLOGY ASSOCIATED TO BIOMECHANICAL STUDIES IN SKELETAL RESEARCH EMPLOYING RAT MODELS [J].
FERRETTI, JL .
BONE, 1995, 17 (04) :S353-S364
[10]
PHYSICAL-ACTIVITY AND BONE MASS - EXERCISES IN FUTILITY [J].
FORWOOD, MR ;
BURR, DB .
BONE AND MINERAL, 1993, 21 (02) :89-112