Sequence variability and functional analysis of MutS of hypermutable Pseudomonas aeruginosa cystic fibrosis isolates

被引：16

作者：

Hogardt, Michael ^{[1
]}

Schubert, Soeren ^{[1
]}

Adler, Kristin ^{[1
]}

Goetzfried, Monika ^{[1
]}

Heesemann, Juergen ^{[1
]}

机构：

[1] Univ Munich, Max Von Pettenkofer Inst Hyg & Med Microbiol, D-80336 Munich, Germany

来源：

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY | 2006年 / 296卷 / 4-5期

关键词：

cystic fibrosis; Pseudomonas aeruginosa; mutator strains; MutS; TGGE;

D O I：

10.1016/j.ijmm.2005.12.018

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study, we investigated the variability of MutS among Pseudomonas aeruginosa recovered from cystic fibrosis (CF) patients. Sequencing of the mutS gene of 15 hypermutable P. aeruginosa isolates obtained from different patients revealed high rates of nucleotide substitutions as compared to that of strain PAO1 Significantly more synonymous than non-synonymous nucleotide substitutions have been found, indicating that generally MutS is highly conserved. The functional analysis of MutS variants by complementation of a PAO1 mutS mutant revealed 5 isolates with a defective MutS due to frameshift mutations or amino acid substitutions. This work supports the hypothesis that the respiratory tract of CF patients represents an environment that favors the selection of highly adaptive mutator phenotypes. (c) 2006 Elsevier GmbH. All rights reserved.

引用

页码：313 / 320

页数：8

共 17 条

[1] Occurrence of hypermutable Pseudomonas aeruginosa in cystic fibrosis patients is associated with the oxidative stress caused by chronic lung inflammation [J].

Ciofu, O ;

Riis, B ;

Pressler, T ;

Poulsen, HE ;

Hoiby, N .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (06) :2276-2282

[2]

*CLSI, 2005, M07A6 CLSI

[3] CLONING AND EXPRESSION IN PSEUDOMONAS-AERUGINOSA OF A GENE INVOLVED IN THE PRODUCTION OF ALGINATE [J].

GOLDBERG, JB ;

OHMAN, DE .

JOURNAL OF BACTERIOLOGY, 1984, 158 (03) :1115-1121

[4] Hypermutation is a key factor in development of multiple-antimicrobial resistance in Pseudomonas aeruginosa strains causing chronic lung infections [J].

Maciá, MD ;

Blanquer, D ;

Togores, B ;

Sauleda, J ;

Pérez, JL ;

Oliver, A .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (08) :3382-3386

[5] Detection and susceptibility testing of hypermutable Pseudomonas aeruginosa strains with the Etest and disk diffusion [J].

Maciá, MD ;

Borrell, N ;

Pérez, JL ;

Oliver, A .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (07) :2665-2672

[6] Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis [J].

Mahenthiralingam, E ;

Campbell, ME ;

Foster, J ;

Lam, JS ;

Speert, DP .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (05) :1129-1135

[7]

MYERS RM, 1985, NUCLEIC ACIDS RES, V13, P3131, DOI 10.1093/nar/13.9.3131

[8]

NEI M, 1986, MOL BIOL EVOL, V3, P418

[9] High frequency of hypermutable Pseudomonas aeruginosa in cystic fibrosis lung infection [J].

Oliver, A ;

Cantón, R ;

Campo, P ;

Baquero, F ;

Blázquez, J .

SCIENCE, 2000, 288 (5469) :1251-1253

[10] The mismatch repair system (mutS, mutL and uvrD genes) in Pseudomonas aeruginosa:: molecular characterization of naturally occurring mutants [J].

Oliver, A ;

Baquero, F ;

Blázquez, J .

MOLECULAR MICROBIOLOGY, 2002, 43 (06) :1641-1650

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