SYBR-Green real-time PCR approach for the detection and quantification of pig DNA in feedstuffs

被引：104

作者：

Martin, Irene ^{[1
]}

Garcia, Teresa ^{[1
]}

Fajardo, Violeta ^{[1
]}

Rojas, Maria ^{[1
]}

Pegels, Nicolette ^{[1
]}

Hernandez, Pablo E. ^{[1
]}

Gonzalez, Isabel ^{[1
]}

Martin, Rosario ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

来源：

MEAT SCIENCE | 2009年 / 82卷 / 02期

关键词：

Real-time PCR; SYBR-Green; Porcine DNA; 12S rRNA; 18S rRNA; Feedstuffs; POLYMERASE-CHAIN-REACTION; IDENTIFICATION; MEAT; QUANTITATION; MIXTURES; PRODUCTS; ASSAY; HEAT; GENE; PORK;

D O I：

10.1016/j.meatsci.2009.01.023

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A real-time polymerase chain reaction assay using primers targeting the porcine-specific mitochondrial 125 rRNA gene and universal eukaryotic primers amplifying a conserved fragment of the nuclear 18S rRNA gene has been developed for the detection and quantification of porcine DNA in food and feedstuffs. The 18S rRNA primers were used as endogenous control for the total content of PCR-amplifiable DNA in the sample. The assay was tested on DNA extracted from raw and heat-treated binary mixtures of porcine tissues in a plant matrix, and on DNA extracted from reference feedstuff samples. Analysis of experimental mixtures demonstrated the suitability of the assay for the detection and quantification of porcine DNA in mixtures containing as little as 0.1%. (c) 2009 Elsevier Ltd. All rights reserved.

引用

页码：252 / 259

页数：8

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