H2S and HS- donor NaHS inhibits intracellular chloride channels

被引：36

作者：

Malekova, Lubica ^{[1
]}

Krizanova, Olga ^{[1
]}

Ondrias, Karol ^{[1
]}

机构：

[1] Slovak Acad Sci, Inst Mol Physiol & Genet, Ctr Excellence Cardiovasc Res, Bratislava 83334, Slovakia

来源：

GENERAL PHYSIOLOGY AND BIOPHYSICS | 2009年 / 28卷 / 02期

关键词：

Chloride channels; H2S; Bilayer lipid membrane; Single channel properties; Heart; HYDROGEN-SULFIDE H2S; NITRIC-OXIDE; MYOCARDIAL-ISCHEMIA; REPERFUSION INJURY; RAT;

D O I：

10.4149/gpb_2009_02_190

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have characterized the effect of H2S on single channel properties of the chloride channels derived from the rat heart lysosomal vesicles incorporated into a bilayer lipid membrane. The single chloride channel currents were measured in 250: 50 mmol/l KCl cis/trans solutions. H2S inhibited the chloride channels by decreasing the channel open probability in a concentration-dependent manner. The inhibitory effect of H2S was side-dependent with the IC50 values of 42 and 75 mu mol/l for the trans and the cis sides, respectively. The mixture of H2S with the NO donor S-nitroso-N-acetyl-DL-penicillamine had smaller effect (IC50 = 180 mu mol/l) than H2S alone. We assume that the inhibitory effect of H2S on chloride channels may be responsible for some of its numerous biological effects.

引用

页码：190 / 194

页数：5

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