Comparison of predicted and experimental subcellular localization of two putative rat steroid dehydrogenases from the short-chain dehydrogenase/reductase protein superfamily

被引：6

作者：

Keller, Brigitte ^{[1
]}

Meier, Marc ^{[1
]}

Adamski, Jerzy ^{[1
,2
]}

机构：

[1] Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Inst Expt Genet, Genome Anal Ctr, D-85764 Neuherberg, Germany

[2] Tech Univ Munich, Lehrstuhl Expt Genet, D-8000 Munich, Germany

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2009年 / 301卷 / 1-2期

关键词：

Subcellular localization; Short-chain dehydrogenases/reductases; SDRs; Dhrs7b; Dhrs8;

D O I：

10.1016/j.mce.2008.07.022

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In the characterization of newly identified proteins, subcellular localization studies can provide important hints to the proteins' metabolic functions. Depending on the biochemical task of an enzyme, certain subcellular environmental conditions as pH or availability of cofactors and substrates have to be fulfilled. Consequently, misdirected proteins often cannot conduct the proper chemical reaction. This study is aimed at detecting differences in bioinformatic and wet lab experiments and presenting ways for reliable analysis of subcellular localization. On a set of ten enzymes from the short-chain dehydrogenase/reductase (SDR) superfamily, we have performed predictions and experimental analyses of subcellular localization. Exemplarily, we show the localization studies on rat short-chain dehydrogenases/reductases dhrs7b and dhrs8. We demonstrate in particular that all of the prediction algorithms tested failed to assign the SDR enzymes to an experimentally verified subcellular compartment. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：43 / 46

页数：4

共 9 条

[1]

Chang NS, 2002, INT J MOL MED, V9, P19

[2] Ribosome binding to mitochondria is regulated by GTP and the transit peptide [J].

Crowley, KS ;

Payne, RM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (27) :17278-17285

[3] Predicting subcellular localization of proteins based on their N-terminal amino acid sequence [J].

Emanuelsson, O ;

Nielsen, H ;

Brunak, S ;

von Heijne, G .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 300 (04) :1005-1016

[4] Support vector machine approach for protein subcellular localization prediction [J].

Hua, SJ ;

Sun, ZR .

BIOINFORMATICS, 2001, 17 (08) :721-728

[5] Bioinformatic identification and characterization of new members of short-chain dehydrogenase/reductase superfamily [J].

Keller, B ;

Volkmann, A ;

Wilckens, T ;

Moeller, G ;

Adamski, J .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2006, 248 (1-2) :56-60

[6] Human 17β-hydroxysteroid dehydrogenases types 1, 2, and 3 catalyze bi-directional equilibrium reactions, rather than unidirectional metabolism, in HEK-293 cells [J].

Khan, N ;

Sharma, KK ;

Andersson, S ;

Auchus, RJ .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2004, 429 (01) :50-59

[7] Sarcolipin regulates the activity of SERCA1, the fast-twitch skeletal muscle sarcoplasmic reticulum Ca2+-ATPase [J].

Odermatt, A ;

Becker, S ;

Khanna, VK ;

Kurzydlowski, K ;

Leisner, E ;

Pette, D ;

MacLennan, DH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (20) :12360-12369

[8] Short-chain dehydrogenases/reductases (SDR): the 2002 update [J].

Oppermann, U ;

Filling, C ;

Hult, M ;

Shafqat, N ;

Wu, XQ ;

Lindh, M ;

Shafqat, J ;

Nordling, E ;

Kallberg, Y ;

Persson, B ;

Jornvall, H .

CHEMICO-BIOLOGICAL INTERACTIONS, 2003, 143 :247-253

[9] Advances in the prediction of protein targeting signals [J].

Schneider, G ;

Fechner, U .

PROTEOMICS, 2004, 4 (06) :1571-1580

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