dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression

被引：44

作者：

Kunert, Natascha ^{[1
]}

Wagner, Eugenia ^{[1
]}

Murawska, Magdalena ^{[1
]}

Klinker, Henrike ^{[1
]}

Kremmer, Elisabeth ^{[2
]}

Brehm, Alexander ^{[1
]}

机构：

[1] Univ Marburg, Inst Mol Biol & Tumorforsch, D-35032 Marburg, Germany

[2] Inst Mol Immunol, Deutsch Forschungszentrum Gesundheit & Umwelt Gmb, Helmholtz Zentrum Munchen, Munich, Germany

来源：

EMBO JOURNAL | 2009年 / 28卷 / 05期

关键词：

ATP-dependent chromatin remodelling; dMEP-1; Mi-2; NuRD; transcription; HISTONE DEACETYLASE; NURD COMPLEX; CELL FATE; DROSOPHILA; BINDING; PROTEIN; DMI-2; MI-2/NURD; MTA3; DERMATOMYOSITIS;

D O I：

10.1038/emboj.2009.3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ATP-dependent chromatin remodeller Mi-2 functions as a transcriptional repressor and contributes to the suppression of cell fates during development in several model organisms. Mi-2 is the ATPase subunit of the conserved Nucleosome Remodeling and Deacetylation (NuRD) complex, and transcriptional repression by Mi-2 is thought to be dependent on its associated histone deacetylase. Here, we have purified a novel dMi-2 complex from Drosophila that is distinct from dNuRD. dMec (dMEP-1 complex) is composed of dMi-2 and dMEP-1. dMec is a nucleosome-stimulated ATPase that is expressed in embryos, larval tissues and adult flies. Surprisingly, dMec is far more abundant than dNuRD and constitutes the major dMi-2-containing complex. Both dNuRD and dMec associate with proneural genes of the achaete-scute complex. However, despite lacking a histone deacetylase subunit, only dMec contributes to the repression of proneural genes. These results reveal an unexpected complexity in the composition and function of Mi-2 complexes.

引用

页码：533 / 544

页数：12

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