Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli

被引：2

作者：

Kim, OG

Kim, IK

Kim, GH

Ko, JS

Park, C

Suh, PG

Kang, SO

Lee, HS ^{[1
]}

Cha, SS

机构：

[1] Pohang Accelerator Lab, Beamline Div, Pohang 790784, Kyungbuk, South Korea

[2] Seoul Natl Univ, Sch Biol Sci, Biophys Lab, Seoul 151742, South Korea

[3] Seoul Natl Univ, Inst Microbiol, Seoul 151742, South Korea

[4] Korea Adv Inst Sci & Technol, Dept Biol Sci, Natl Creat Res Initiat Ctr Behav Genet, Taejon 305710, South Korea

[5] Pohang Univ Sci & Technol, Dept Life Sci, Div Mol & Life Sci, Pohang 790784, South Korea

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2002年 / 58卷

关键词：

D O I：

10.1107/S0907444902007369

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A yedU gene product with a molecular mass of 31 kDa is a hypothetical protein with no known function. The protein was purified and crystallized at 296 K. X-ray diffraction data have been collected to 2.3 Angstrom using synchrotron radiation. The crystals belong to the primitive orthorhombic system, with unit-cell parameters a = 50.56, b = 63.45, c = 168.02 Angstrom. The asymmetric unit contains two monomers of the protein, with a corresponding V-M of 2.25 Angstrom(3) Da(-1) and a solvent content of 44.84%.

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页码：1217 / 1219

页数：3

相关论文

共 33 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] The InterPro database, an integrated documentation resource for protein families, domains and functional sites [J].

Apweiler, R ;

Attwood, TK ;

Bairoch, A ;

Bateman, A ;

Birney, E ;

Biswas, M ;

Bucher, P ;

Cerutti, T ;

Corpet, F ;

Croning, MDR ;

Durbin, R ;

Falquet, L ;

Fleischmann, W ;

Gouzy, J ;

Hermjakob, H ;

Hulo, N ;

Jonassen, I ;

Kahn, D ;

Kanapin, A ;

Karavidopoulou, Y ;

Lopez, R ;

Marx, B ;

Mulder, NJ ;

Oinn, TM ;

Pagni, M ;

Servant, F ;

Sigrist, CJA ;

Zdobnov, EM .

NUCLEIC ACIDS RESEARCH, 2001, 29 (01) :37-40

[3] The histone-like protein H-NS acts as a transcriptional repressor for expression of the anaerobic and growth phase activator AppY of Escherichia coli [J].

Atlung, T ;

Sund, S ;

Olesen, K ;

Brondsted, L .

JOURNAL OF BACTERIOLOGY, 1996, 178 (12) :3418-3425

[4] H-NS: A modulator of environmentally regulated gene expression [J].

Atlung, T ;

Ingmer, H .

MOLECULAR MICROBIOLOGY, 1997, 24 (01) :7-17

[5] Pfam 3.1: 1313 multiple alignments and profile HMMs match the majority of proteins [J].

Bateman, A ;

Birney, E ;

Durbin, R ;

Eddy, SR ;

Finn, RD ;

Sonnhammer, ELL .

NUCLEIC ACIDS RESEARCH, 1999, 27 (01) :260-262

[6] MUTATIONS IN BGLY, THE STRUCTURAL GENE FOR THE DNA-BINDING PROTEIN H1, AFFECT EXPRESSION OF SEVERAL ESCHERICHIA-COLI GENES [J].

BERTIN, P ;

LEJEUNE, P ;

LAURENTWINTER, C ;

DANCHIN, A .

BIOCHIMIE, 1990, 72 (12) :889-891

[7] CRYSTAL-STRUCTURE OF CATALASE HPII FROM ESCHERICHIA-COLI [J].

BRAVO, J ;

VERDAGUER, N ;

TORMO, J ;

BETZEL, C ;

SWITALA, J ;

LOEWEN, PC ;

FITA, I .

STRUCTURE, 1995, 3 (05) :491-502

[8] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[9] Promoter-specific repression of fimB expression by the Escherichia coli nucleoid-associated protein H-NS [J].

Donato, GM ;

Lelivelt, MJ ;

Kawula, TH .

JOURNAL OF BACTERIOLOGY, 1997, 179 (21) :6618-6625

[10] Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-Å resolution [J].

Du, XL ;

Choi, IG ;

Kim, R ;

Wang, WR ;

Jancarik, J ;

Yokota, H ;

Kim, SH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (26) :14079-14084

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