New and simple procedure for the determination of muramic acid in chemically complex environments by gas chromatography-ion trap tandem mass spectrometry

被引：25

作者：

Bal, K ^{[1
]}

Larsson, L ^{[1
]}

机构：

[1] Lund Univ, Dept Infect Dis & Med Microbiol, Sect Bacteriol, S-22362 Lund, Sweden

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2000年 / 738卷 / 01期

关键词：

muramic acid;

D O I：

10.1016/S0378-4347(99)00494-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gas chromatographic-ion trap tandem mass spectrometric method was developed for the quantification of muramic acid, a marker of bacterial peptidoglycan, in environmental and clinical specimens. Samples (bacteria, house dust and urine) were heated in methanolic hydrochloric acid overnight and extracted with hexane for removal of hydrophobic compounds. The aqueous phase was evaporated and heated in acetic anhydride and pyridine after which the product, the acetate derivative, was washed with dilute hydrochloric acid and water. The described method is both rapid and simple to apply, and produces a stable derivative. It should become widely used for measuring peptidoglycan in chemically complex environments. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：57 / 65

页数：9

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