Comparison of PCR-based methods for typing Staphylococcus aureus isolates

被引：51

作者：

Sabat, Artur

Malachowa, Natalia

Miedzobrodzki, Jacek

Hryniewicz, Waleria

机构：

[1] Natl Inst Publ Hlth, PL-00725 Warsaw, Poland

[2] Jagiellonian Univ, Dept Microbiol, Fac Biotechnol, PL-30387 Krakow, Poland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 10期

关键词：

D O I：

10.1128/JCM.00395-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study, we compared the potentials of (i) a multiplex PCR-based multilocus variable-number tandem repeat (VNTR) assay; (ii) a triplex PCR coamplifying fragments of spa, coa, and the hypervariable region adjacent to the mecA gene; (iii) restriction profile analysis of the STAR repetitive element; (iv) randomly amplified polymorphic DNA analysis; (v) inter-IS256 PCR; and (vi) rep-MP3 PCR. Multilocus VNTR typing and triplex PCR (coa, spa, and hypervariable region) approaches showed excellent reproducibility and high discriminatory power; however, only multilocus VNTR typing could distinguish all pulsed-field gel electrophoresis and spa types. Multilocus VNTR typing appears to be the most useful PCR-based method for the rapid genotyping of Staphylococcus aureus strains.

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收藏

页码：3804 / 3807

页数：4

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