Asymmetric sulfoxidation and amine binding by H64D/V68A and H64D/V68S Mb: Mechanistic insight into the chiral discrimination step

被引:34
作者
Kato, S
Yang, HJ
Ueno, T
Ozaki, S
Phillips, GN
Fukuzumi, S
Watanabe, Y [1 ]
机构
[1] Nagoya Univ, Grad Sch Sci, Dept Chem, Nagoya, Aichi 4648602, Japan
[2] Grad Univ Adv Studies, Dept Struct Mol Sci, Okazaki, Aichi 4448585, Japan
[3] Yamagata Univ, Fac Educ, Yamagata 9908560, Japan
[4] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA
[5] Osaka Univ, JST, CREST, Grad Sch Engn,Dept Mat & Life Sci, Suita, Osaka 5650871, Japan
关键词
D O I
10.1021/ja0256414
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The H64D/V68A and H64D/V68S mutants of Myoglobin are found to oxidize thioanisole with high enantioselectivity and reactivity. These mutants are also capable of enantioselective binding of α-methylbenzylamine, which mimics an expected sulfoxidation intermediate. The kinetic study of the amine binding shows that the Fe-O bond cleavage in the intermediate may be the chiral discrimination step of the sulfoxidation. Copyright © 2002 American Chemical Society.
引用
收藏
页码:8506 / 8507
页数:2
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