Asymmetric sulfoxidation and amine binding by H64D/V68A and H64D/V68S Mb: Mechanistic insight into the chiral discrimination step

被引：34

作者：

Kato, S

Yang, HJ

Ueno, T

Ozaki, S

Phillips, GN

Fukuzumi, S

Watanabe, Y ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Sci, Dept Chem, Nagoya, Aichi 4648602, Japan

[2] Grad Univ Adv Studies, Dept Struct Mol Sci, Okazaki, Aichi 4448585, Japan

[3] Yamagata Univ, Fac Educ, Yamagata 9908560, Japan

[4] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

[5] Osaka Univ, JST, CREST, Grad Sch Engn,Dept Mat & Life Sci, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 29期

关键词：

D O I：

10.1021/ja0256414

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The H64D/V68A and H64D/V68S mutants of Myoglobin are found to oxidize thioanisole with high enantioselectivity and reactivity. These mutants are also capable of enantioselective binding of α-methylbenzylamine, which mimics an expected sulfoxidation intermediate. The kinetic study of the amine binding shows that the Fe-O bond cleavage in the intermediate may be the chiral discrimination step of the sulfoxidation. Copyright © 2002 American Chemical Society.

引用

页码：8506 / 8507

页数：2

共 17 条

[1] ROLES OF PROXIMAL LIGAND IN HEME-PROTEINS - REPLACEMENT OF PROXIMAL HISTIDINE OF HUMAN MYOGLOBIN WITH CYSTEINE AND TYROSINE BY SITE-DIRECTED MUTAGENESIS AS MODELS FOR P-450, CHLOROPEROXIDASE, AND CATALASE [J].