Combination of conventional blood cultures and the SeptiFast molecular test in patients with suspected sepsis for the identification of bloodstream pathogens

被引：22

作者：

Burdino, Elisa ^{[1
]}

Ruggiero, Tina ^{[1
]}

Allice, Tiziano ^{[1
]}

Milia, Maria Grazia ^{[1
]}

Gregori, Gabriella ^{[1
]}

Milano, Rosangela ^{[1
]}

Cerutti, Francesco ^{[1
]}

De Rosa, Francesco Giuseppe ^{[2
]}

Manno, Emilpaolo ^{[3
,4
]}

Caramello, Pietro ^{[2
]}

Di Perri, Giovanni ^{[2
]}

Ghisetti, Valeria ^{[1
]}

机构：

[1] Amedeo di Savoia Hosp, Dept Infect Dis, Lab Microbiol & Virol, I-10149 Turin, Italy

[2] Univ Turin, Amedeo di Savoia Hosp, Dept Infect Dis, I-10149 Turin, Italy

[3] Maria Vittoria Hosp, Emergency Dept, I-10149 Turin, Italy

[4] Maria Vittoria Hosp, Intens Care Unit, I-10149 Turin, Italy

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2014年 / 79卷 / 03期

关键词：

Sepsis; SeptiFast; Bloodstream infection; Blood culture; REAL-TIME PCR; ANTIMICROBIAL THERAPY; LIGHTCYCLER SEPTIFAST; FUNGAL PATHOGENS; RAPID DETECTION; DIAGNOSIS; ASSAY; INFECTIONS; BACTERIAL; SHOCK;

D O I：

10.1016/j.diagmicrobio.2014.03.018

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We evaluated performances of the molecular test SeptiFast (SF) for the detection of agents of bloodstream infection (BSI) in patients with suspected sepsis, the majority of them under antibiotic treatment and at high prevalence of HIV-1 infection (10.5%). Matched SF and blood culture (BC) samples (n = 1186) from 1024 patients were studied. Two hundred fifty-one episodes of BSI out of 1144 were identified with the combined methods (22%). SF identified more episodes of BSI than BC: 206 versus 176 (chi(2) = 7.008, P = 0.0081) and a significantly higher number of Gram-negative bacteria than BC (77 versus 53, chi(2) = 9.12; P = 0.0025), as well as of polymicrobial infections (chi(2) = 4.50, P = 0.0339). In conclusion, SF combined with BC improved the diagnosis of sepsis, especially in immunocompromised patients. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：287 / 292

页数：6

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