Lamellar organization of pigments in chlorosomes, the light harvesting complexes of green photosynthetic bacteria

被引:187
作者
Psencík, J
Ikonen, TP
Laurinmäki, P
Merckel, MC
Butcher, SJ
Serimaa, RE
Tuma, R
机构
[1] Univ Helsinki, Inst Biotechnol, Helsinki Bioenerget Grp, FIN-00014 Helsinki, Finland
[2] Univ Helsinki, Dept Phys Sci, Div Xray Phys, FIN-00014 Helsinki, Finland
[3] Univ Helsinki, Dept Biol & Environm Sci, FIN-00014 Helsinki, Finland
[4] Charles Univ, Fac Math & Phys, Dept Chem Phys & Opt, Prague, Czech Republic
基金
芬兰科学院;
关键词
D O I
10.1529/biophysj.104.040956
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Chlorosomes of green photosynthetic bacteria constitute the most efficient light harvesting complexes found in nature. In addition, the chlorosome is the only known photosynthetic system where the majority of pigments (BChl) is not organized in pigment-protein complexes but instead is assembled into aggregates. Because of the unusual organization, the chlorosome structure has not been resolved and only models, in which BChl pigments were organized into large rods, were proposed on the basis of freeze-fracture electron microscopy and spectroscopic constraints. We have obtained the first high-resolution images of chlorosomes from the green sulfur bacterium Chlorobium tepidum by cryoelectron microscopy. Cryoelectron microscopy images revealed dense striations similar to20 Angstrom apart. X-ray scattering from chlorosomes exhibited a feature with the same similar to20 AAngstrom spacing. No evidence for the rod models was obtained. The observed spacing and tilt-series cryoelectron microscopy projections are compatible with a lamellar model, in which BChl molecules aggregate into semicrystalline lateral arrays. The diffraction data further indicate that arrays are built from BChl dimers. The arrays form undulating lamellae, which, in turn, are held together by interdigitated esterifying alcohol tails, carotenoids, and lipids. The lamellar model is consistent with earlier spectroscopic data and provides insight into chlorosome self-assembly.
引用
收藏
页码:1165 / 1172
页数:8
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