Effects of environmental factors on two-stage tannin-protein co-precipitation

被引：42

作者：

Kawamoto, H ^{[1
]}

Nakatsubo, F ^{[1
]}

机构：

[1] KYOTO UNIV,GRAD SCH AGR,SAKYO KU,KYOTO 60601,JAPAN

来源：

PHYTOCHEMISTRY | 1997年 / 46卷 / 03期

关键词：

tannin; galloylglucose; protein-precipitation; two-stage mechanism; pH; temperature; concentration; ionic strength;

D O I：

10.1016/S0031-9422(97)00333-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Effects of environmental factors on two-stage co-precipitation of tetragalloylglucose with three different proteins [bovine serum albumin (BSA), lysozyme, and myoglobin] were investigated. Factors such as pH, temperature, and ionic strength, mainly affected the second precipitation stage. On the other hand, protein concentration mainly affected the initial complexation stage. Precipitability of the galloylglucose-protein complexes is directly related to the solubility of the original protein under each environmental condition. (C) 1997 Elsevier Science Ltd.

引用

页码：479 / 483

页数：5

共 19 条

[1]

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[2]

GOLDSTEIN JUDITH L., 1965, PHYTOCHEMISTRY, V4, P185, DOI 10.1016/S0031-9422(00)86162-2

[3] INTERACTION OF VEGETABLE TANNINS WITH POLYAMIDES AS PROOF OF THE DOMINANT FUNCTION OF THE PEPTIDE BOND OF COLLAGEN FOR ITS BINDING OF TANNINS [J].