Differential display proteomic analysis of Picea meyeri pollen germination and pollen-tube growth after inhibition of actin polymerization by latrunculin B

被引:62
作者
Chen, Yanmei
Chen, Tong
Shen, Shihua
Zheng, Maozhong
Guo, Yiming
Lin, Jinxing [1 ]
Baluska, Frantisek
Samaj, Jozef
机构
[1] Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Mol Environm Physiol, Beijing 100093, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
[3] Univ Bonn, Inst Cellular & Mol Bot, D-53115 Bonn, Germany
[4] Slovak Acad Sci, Inst Bot, SK-842263 Bratislava, Slovakia
[5] Slovak Acad Sci, Inst Plant Genet & Biotechnol, SK-95007 Nitra, Slovakia
关键词
actin cytoskeleton; tip growth; proteomics; mass spectrometry; Picea meyeri; pollen tube;
D O I
10.1111/j.1365-313X.2006.02783.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To investigate roles of the actin cytoskeleton in growth of the pollen tube of Picea meyeri, we used the actin polymerization inhibitor latrunculin B (LATB) under quantitatively controlled conditions. At low concentrations, LATB inhibited polymerization of the actin cytoskeleton in the growing pollen tube, which rapidly inhibited tip growth. The proteomic approach was used to analyse protein expression-profile changes during pollen germination and subsequent pollen-tube development with disturbed organization of the actin cytoskeleton. Two-dimensional electrophoresis and staining with Coomassie Brilliant Blue revealed nearly 600 protein spots. A total of 84 of these were differentially displayed at different hours with varying doses of LATB, and 53 upregulated or downregulated proteins were identified by mass spectrometry. These proteins were grouped into distinct functional categories including signalling, actin cytoskeleton organization, cell expansion and carbohydrate metabolism. Moreover, actin disruption affected the morphology of Golgi stacks, mitochondria and amyloplasts, along with a differential expression of proteins involved in their functions. These findings provide new insights into the multifaceted mechanism of actin cytoskeleton functions and its interaction with signalling, cell-expansion machinery and energy-providing pathways.
引用
收藏
页码:174 / 195
页数:22
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