Detection of protein-ligand interaction on the membranes using C-terminus biotin-tagged alamethicin

被引：17

作者：

Zhang, Y ^{[1
]}

Futaki, S ^{[1
]}

Kiwada, T ^{[1
]}

Sugiura, Y ^{[1
]}

机构：

[1] Kyoto Univ, Inst Chem Res, Uji, Kyoto 6110011, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2002年 / 10卷 / 08期

关键词：

D O I：

10.1016/S0968-0896(02)00105-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C-terminal biotin-tagged alamethicin, which has several alpha-aminoisobutyric acid (Aib) residues in its sequence, was synthesized by the preparation of the protected peptide segment using the 2-chlorotrityl resin, followed by conjugation with biotin hydrazide. Suppression of the channel current of the biotin-tagged alamethicin by the addition of streptavidin to the electrolyte was monitorable in real time using the planar lipid-bilayer method. The system was also applicable to the detection of interaction of the biotin-tagged alamethicin with the anti-biotin antibody. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：2635 / 2639

页数：5

共 36 条

[1] SYNTHETIC PEPTIDES AS MODELS FOR ION CHANNEL PROTEINS [J].