Crystallization and preliminary crystallographic study of triple-helical DNA

被引：4

作者：

Han, ZJ ^{[1
]}

Rhee, S ^{[1
]}

Liu, KL ^{[1
]}

Miles, HT ^{[1
]}

Davies, DR ^{[1
]}

机构：

[1] NIDDK, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2000年 / 56卷

关键词：

D O I：

10.1107/S0907444999012895

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single crystals of d(CTCCT(S)CCGCGCG).d(CGCGCGGAG) have been grown by the vapor-diffusion method using 2-methyl-2,4-pentanediol as a precipitant. The crystals are tetragonal, space group P4(2), with unit-cell parameters a = b = 53.8, c = 43.1 Angstrom, and diffract to 1.8 Angstrom resolution at a synchrotron X-ray beamline. In the crystal, the asymmetric unit contains one copy of the construct. The two halves of the structure are related by non-crystallographic twofold symmetry. These observations are consistent with the conclusion that the sequences of the 12-mer and 9-mer oligonucleotides form a duplex DNA at one end and a tripler DNA at the other end.

引用

页码：104 / 105

页数：2

共 12 条

[1] A NUCLEIC-ACID TRIPLE-HELIX FORMED BY A PEPTIDE NUCLEIC-ACID DNA COMPLEX [J].