A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis

被引：85

作者：

Biery, MC

Stewart, FJ

Stellwagen, AE

Raleigh, EA

Craig, NL

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Howard Hughes Med Inst, Baltimore, MD 21205 USA

[2] New England Biolabs Inc, Beverly, MA 01915 USA

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 05期

关键词：

D O I：

10.1093/nar/28.5.1067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A robust Tn7-based in vitro transposition system is described that displays little target site selectivity, allowing the efficient recovery of many different transposon insertions in target DNAs ranging from small plasmids to cosmids to whole genomes. Two miniTn7 derivatives are described that are useful for the analysis of genes: one a derivative for making translational and transcriptional target gene fusions and the other a derivative that can generate 15 bp (5 amino acid) insertions in target DNAs (proteins).

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页码：1067 / 1077

页数：11

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