Nitrite concentration influences the population structure of Nitrospira-like bacteria

被引:175
作者
Maixner, Frank
Noguera, Daniel R.
Anneser, Bettina
Stoecker, Kilian
Wegl, Gertrude
Wagner, Michael
Daims, Holger
机构
[1] Univ Vienna, Dept Mikrobielle Okol, A-1090 Vienna, Austria
[2] Univ Wisconsin, Dept Civil & Environm Engn, Madison, WI 53706 USA
关键词
D O I
10.1111/j.1462-2920.2006.01033.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Chemolithoautotrophic nitrite oxidizers of the genus Nitrospira are a monophyletic but diverse group of organisms, are widely distributed in many natural habitats, and play a key role in nitrogen elimination during biological wastewater treatment. Phylogenetic analyses of cloned 16S rRNA genes and fluorescence in situ hybridization with newly developed rRNA-targeted oligonucleotide probes revealed coexistence of uncultured members of sublineages I and II of the genus Nitrospira in biofilm and activated sludge samples taken from nitrifying wastewater treatment plants. Quantitative microscopic analyses of their spatial arrangement relative to ammonia oxidizers in the biofilm and activated sludge flocs showed that members of the Nitrospira sublineage I occurred significantly more often in immediate vicinity to ammonia oxidizers than would be expected from random community assembly while such a relationship was not observed for Nitrospira sublineage II. This spatial distribution suggested a niche differentiation of these coexisting Nitrospira populations with respect to their preferred concentrations of nitrite. This hypothesis was tested by mathematical modelling of nitrite consumption and resulting nitrite gradients in nitrifying biofilms and by quantifying the abundance of sublineage I and II Nitrospira in activated sludge during incubations with nitrite in different concentrations. Consistent with the observed localization patterns, a higher nitrite concentration selected for sublineage I but suppressed sublineage II Nitrospira.
引用
收藏
页码:1487 / 1495
页数:9
相关论文
共 43 条
[1]   The oligonucleotide probe database [J].
Alm, EW ;
Oerther, DB ;
Larsen, N ;
Stahl, DA ;
Raskin, L .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (10) :3557-3559
[2]   COMBINATION OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES WITH FLOW-CYTOMETRY FOR ANALYZING MIXED MICROBIAL-POPULATIONS [J].
AMANN, RI ;
BINDER, BJ ;
OLSON, RJ ;
CHISHOLM, SW ;
DEVEREUX, R ;
STAHL, DA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (06) :1919-1925
[3]  
[Anonymous], 1996, ECOLOGY
[4]   Application of activated sludge and biofilm sequencing batch reactor technology to treat reject water from sludge dewatering systems:: a comparison [J].
Arnold, E ;
Böhm, B ;
Wilderer, PA .
WATER SCIENCE AND TECHNOLOGY, 2000, 41 (01) :115-122
[5]   Activated sludge flocculation: On-line determination of floc size and the effect of shear [J].
Biggs, CA ;
Lant, PA .
WATER RESEARCH, 2000, 34 (09) :2542-2550
[6]   GENE ORGANIZATION AND PRIMARY STRUCTURE OF A RIBOSOMAL-RNA OPERON FROM ESCHERICHIA-COLI [J].
BROSIUS, J ;
DULL, TJ ;
SLEETER, DD ;
NOLLER, HF .
JOURNAL OF MOLECULAR BIOLOGY, 1981, 148 (02) :107-127
[7]  
Burrell PC, 1998, APPL ENVIRON MICROB, V64, P1878
[8]   daime, a novel image analysis program for microbial ecology and biofilm research [J].
Daims, H ;
Lücker, S ;
Wagner, M .
ENVIRONMENTAL MICROBIOLOGY, 2006, 8 (02) :200-213
[9]   The domain-specific probe EUB338 is insufficient for the detection of all Bacteria:: Development and evaluation of a more comprehensive probe set [J].
Daims, H ;
Brühl, A ;
Amann, R ;
Schleifer, KH ;
Wagner, M .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1999, 22 (03) :434-444
[10]   In situ characterization of Nitrospira-like nitrite oxidizing bacteria active in wastewater treatment plants [J].
Daims, H ;
Nielsen, JL ;
Nielsen, PH ;
Schleifer, KH ;
Wagner, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (11) :5273-5284