Development of a screening method to determine the pattern of fermentation metabolites in faecal samples using on-line purge-and-trap gas chromatographic-mass spectrometric analysis

被引：54

作者：

De Preter, Vicky ^{[1
,2
]}

Van Staeyen, Greet ^{[1
,2
]}

Esser, Diederik ^{[1
,2
]}

Rutgeerts, Paul ^{[1
,2
]}

Verbeke, Kristin ^{[1
,2
]}

机构：

[1] Katholieke Univ Leuven Hosp, Dept Gastrointestinal Res, B-3000 Louvain, Belgium

[2] Katholieke Univ Leuven Hosp, Leuven Food Sci & Nutr Res Ctr, B-3000 Louvain, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 09期

关键词：

Volatile organic compounds; Purge-and-trap; GC-TOF-MS; SOLID-PHASE MICROEXTRACTION; HUMAN LARGE-INTESTINE; CHAIN FATTY-ACIDS; BACTERIAL METABOLITES; HYDROGEN METABOLISM; HEALTHY-VOLUNTEERS; MARKERS RELEVANT; DIETARY-PROTEIN; HUMAN-COLON; IN-VITRO;

D O I：

10.1016/j.chroma.2008.12.095

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

An on-line screening method to analyse volatile organic compounds (VOCs) in faecal samples was developed. VOCs were isolated from a standard solution or faecal samples using a purge-and-trap system and identified and quantified by GC-MS. The experimental conditions were optimised and the performance of the system was evaluated. Linear calibration curves were obtained with correlation coefficients of at least 0.992. RSDs within and between days were less than 10%. The method was successfully applied to the analysis of faecal samples, yielding 135 different volatile organic compounds identified in 11 faecal samples. Of those, 22 VOCs were found in all volunteers, whereas 34 VOCs were person-specific. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1476 / 1483

页数：8

共 35 条

[1]

Comparison of volatiles, phenolics, sugars, antioxidant vitamins, and sensory quality of different colored carrot varieties [J].

Alasalvar, C ;

Grigor, JM ;

Zhang, DL ;

Quantick, PC ;

Shahidi, F .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (03) :1410-1416

[2]

Composition and metabolism of the intestinal microbiota in consumers and non-consumers of yogurt [J].

Alvaro, Elise ;

Andrieux, Claude ;

Rochet, Violaine ;

Rigottier-Gois, Lionel ;

Lepercq, Pascale ;

Sutren, Malene ;

Galan, Pilar ;

Duval, Yvonne ;

Juste, Catherine ;

Dore, Joel .

BRITISH JOURNAL OF NUTRITION, 2007, 97 (01) :126-133

[3]

ARMBRUSTER DA, 1994, CLIN CHEM, V40, P1233

[4]

Resistant starch lowers fecal concentrations of ammonia and phenols in humans [J].

Birkett, A ;

Muir, J ;

Phillips, J ;

Jones, G ;

ODea, K .

AMERICAN JOURNAL OF CLINICAL NUTRITION, 1996, 63 (05) :766-772

[5]

Whole-grain wheat breakfast cereal has a prebiotic effect on the human gut microbiota: a double-blind, placebo-controlled, crossover study [J].

Costabile, Adele ;

Klinder, Annett ;

Fava, Francesca ;

Napolitano, Aurora ;

Fogliano, Vincenzo ;

Leonard, Clare ;

Gibson, Glenn R. ;

Tuohy, Kieran M. .

BRITISH JOURNAL OF NUTRITION, 2008, 99 (01) :110-120

[6]

Cummings JH, 2001, AM J CLIN NUTR, V73, p415S, DOI 10.1093/ajcn/73.2.415s

[7]

SHORT CHAIN FATTY-ACIDS IN THE HUMAN-COLON [J].

CUMMINGS, JH .

GUT, 1981, 22 (09) :763-779

[8]

EFFECT OF MEAT PROTEIN AND DIETARY FIBER ON COLONIC FUNCTION AND METABOLISM .2. BACTERIAL METABOLITES IN FECES AND URINE [J].

CUMMINGS, JH ;

HILL, MJ ;

BONE, ES ;

BRANCH, WJ ;

JENKINS, DJA .

AMERICAN JOURNAL OF CLINICAL NUTRITION, 1979, 32 (10) :2094-2101

[9]

Influence of long-term administration of lactulose and Saccharomyces boulardii on the colonic generation of phenolic compounds in healthy human subjects [J].

De Preter, Vicky ;

Coopmans, Tamara ;

Rutgeerts, Paul ;

Verbeke, Kristin .

JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION, 2006, 25 (06) :541-549

[10]

ENERGY-METABOLISM OF MEDIUM-CHAIN TRIGLYCERIDES VERSUS CARBOHYDRATES DURING EXERCISE [J].

DECOMBAZ, J ;

ARNAUD, MJ ;

MILON, H ;

MOESCH, H ;

PHILIPPOSSIAN, G ;

THELIN, AL ;

HOWALD, H .

EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY AND OCCUPATIONAL PHYSIOLOGY, 1983, 52 (01) :9-14

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