Immobilization of pancreatic islet cells with preserved secretory potential

被引：6

作者：

Malaisse, WJ

Olivares, E

Belcourt, A

Nilsson, K

机构：

[1] Free Univ Brussels, Expt Med Lab, B-1070 Brussels, Belgium

[2] Ctr European Etude Diabet, Strasbourg, France

[3] Percell Biolytica, Astorp, Sweden

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1999年 / 52卷 / 05期

关键词：

Immobilization; Gelatin; Theophylline; Islet Cell; Pancreatic Islet;

D O I：

10.1007/s002530051573

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Dispersed pancreatic islet cells from rats were cultured overnight in the presence of macroporous gelatin microcarriers. The cells attached to the microcarriers were then incubated for 90 min in the absence or presence of 15.0 mM D-glucose and/or 1.25 mM theophylline. The release of insulin during incubation was about three times higher in the simultaneous presence of these two secretagogues than in their absence. This procedure could thus be used for the immobilization of pancreatic islet cells with preserved secretory potential.

引用

页码：652 / 653

页数：2

共 5 条

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