Bacterial Polyhydroxyalkanoate Granules: Biogenesis, Structure, and Potential Use as Nano-/Micro-Beads in Biotechnological and Biomedical Applications

被引：182

作者：

Grage, Katrin ^{[1
]}

Jahns, Anika C. ^{[1
]}

Parlane, Natalie ^{[1
,2
]}

Palanisamy, Rajasekaran ^{[1
]}

Rasiah, Indira A. ^{[1
]}

Atwood, Jane A. ^{[1
]}

Rehm, Bernd H. A. ^{[1
]}

机构：

[1] Massey Univ, Inst Mol Biosci, Palmerston North 4442, New Zealand

[2] Massey Univ, Hopkirk Res Inst, Palmerston North 4442, New Zealand

来源：

BIOMACROMOLECULES | 2009年 / 10卷 / 04期

关键词：

POLY-BETA-HYDROXYBUTYRATE; RALSTONIA-EUTROPHA H16; MEDIUM-CHAIN-LENGTH; PHA INCLUSION-BODIES; IN-VIVO; ESCHERICHIA-COLI; PSEUDOMONAS-AERUGINOSA; BIOSYNTHESIS GENES; POLY(3-HYDROXYBUTYRATE) PHB; ALCALIGENES-EUTROPHUS;

D O I：

10.1021/bm801394s

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyhydroxyalkanoates (PHAs) are naturally occurring organic polyesters that are of interest for industrial and biomedical applications. These polymers are synthesized by most bacteria in times of unbalanced nutrient availability from a variety of substrates and they are deposited intracellularly as insoluble spherical inclusions or PHA granules. The granules consist of a polyester core, surrounded by a boundary layer with embedded or attached proteins that include the PHA synthase, phasins, depolymerizing enzymes, and regulatory proteins. Apart from ongoing industrial interest in the material PHA, more recently there has also been increasing interest in applications of the PHA granules as nano-/micro-beads after it was conceived that fusions to the granule associated proteins (GAPs) provide a way to immobilize target proteins at the granule surface. This review gives an overview of PHA granules in general, including biogenesis and GAPs, and focuses on their potential use as nano-/micro-beads in biotechnological and biomedical applications.

引用

页码：660 / 669

页数：10

共 139 条

[1] Replacement of the catalytic nucleophile cysteine-296 by serine in class II polyhydroxyalkanoate synthase from Pseudomonas aeruginosa-mediated synthesis of a new polyester:: identification of catalytic residues [J].

Amara, AA ;

Rehm, BHA .

BIOCHEMICAL JOURNAL, 2003, 374 :413-421

[2] OCCURRENCE, METABOLISM, METABOLIC ROLE, AND INDUSTRIAL USES OF BACTERIAL POLYHYDROXYALKANOATES [J].

ANDERSON, AJ ;

DAWES, EA .

MICROBIOLOGICAL REVIEWS, 1990, 54 (04) :450-472

[3] Protein engineering towards biotechnological production of bifunctional polyester beads [J].

Atwood, Jane A. ;

Rehm, Bernd H. A. .

BIOTECHNOLOGY LETTERS, 2009, 31 (01) :131-137

[4] Recombinant Escherichia coli produces tailor-made biopolyester granules for applications in fluorescence activated cell sorting:: functional display of the mouse interleukin-2 and myelin oligodendrocyte glycoprotein [J].

Backstrom, B. Thomas ;

Brockelbank, Jane A. ;

Rehm, Bernd H. A. .

BMC BIOTECHNOLOGY, 2007, 7 (1)

[5] Novel and economical purification of recombinant proteins: Intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association [J].

Banki, MR ;

Gerngross, TU ;

Wood, DW .

PROTEIN SCIENCE, 2005, 14 (06) :1387-1395

[6]

BANKI MR, 2005, MICROB CELL FACT, P4

[7] Integrated recombinant protein expression and purification platform based on Ralstonia eutropha [J].

Barnard, GC ;

McCool, JD ;

Wood, DW ;

Gerngross, TU .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (10) :5735-5742

[8]

BARNARD GN, 1989, J BIOL CHEM, V264, P3286

[9] PSEUDOMONAS-OLEOVORANS AS A SOURCE OF POLY(BETA-HYDROXYALKANOATES) FOR POTENTIAL APPLICATIONS AS BIODEGRADABLE POLYESTERS [J].

BRANDL, H ;

GROSS, RA ;

LENZ, RW ;

FULLER, RC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (08) :1977-1982

[10] Recombinant Escherichia coli strain produces a ZZ domain displaying biopolyester granules suitable for immunoglobulin G purification [J].

Brockelbank, Jane A. ;

Peters, Verena ;

Rehm, Bernd H. A. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (11) :7394-7397

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