Extending the Range of Microsecond-to-Millisecond Chemical Exchange Detected in Labeled and Unlabeled Nucleic Acids by Selective Carbon R1ρ NMR Spectroscopy

被引：92

作者：

Hansen, Alexandar L. ^{[1
]}

Nikolova, Evgenia N. ^{[1
]}

Casiano-Negroni, Anette ^{[1
]}

Al-Hashimi, Hashim M. ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem & Biophys, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 11期

基金：

美国国家科学基金会;

关键词：

TRANSITION CROSS-POLARIZATION; MAGNETIC-RESONANCE; RIBOSOMAL-RNA; DYNAMICS; RELAXATION; FIELDS; TIME; SITE; DNA;

D O I：

10.1021/ja8091399

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present an off-resonance carbon R-1 rho NMR experiment utilizing weak radiofrequency fields and selective polarization transfers for quantifying chemical-exchange processes in nucleic acids. The experiment extends the range of accessible time scales to similar to 10 ms, and its time-saving feature makes it possible to thoroughly map out dispersion profiles and conduct measurements at natural abundance. The experiment unveiled microsecond-to-millisecond exchange dynamics in a uniformly labeled A-site rRNA and in unlabeled, damaged DNA that would otherwise be difficult to characterize by conventional methods.

引用

页码：3818 / +

页数：3

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