Immunoquantitation of cytochromes P450 1A and P450 2B and comparison with chlorinated hydrocarbon levels in archived polar bear liver samples

被引：25

作者：

Bandiera, SM ^{[1
]}

Torok, SM ^{[1
]}

Letcher, RJ ^{[1
]}

Norstrom, RJ ^{[1
]}

机构：

[1] CANADIAN WILDLIFE SERV, NATL WILDLIFE RES CTR, HULL, PQ K1A 0H3, CANADA

来源：

CHEMOSPHERE | 1997年 / 34卷 / 5-7期

关键词：

D O I：

10.1016/S0045-6535(97)00443-8

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The present study examined the utility of an immunoblot method for quantitation of cytochrome P450 isozymes in archived liver samples as a bioassay of exposure to halogenated hydrocarbons. Hepatic microsomes were prepared from 44 archived polar bear (Ursus maritimus) liver homogenates that had been stored at approximately -40 degrees C for 9-10 years and analyzed on blots probed with antibodies to rat cytochromes P450 1A1 and P450 2B1. The results revealed a positive correlation between cytochrome P450 1A and total polychlorinated biphenyl (PCB) levels in the archived liver samples, suggesting that cytochrome P450 1A was induced in polar bears by environmental exposure to PCBs. (C) 1997 Elsevier Science Ltd.

引用

页码：1469 / 1479

页数：11

共 13 条

[1] ORGANOCHLORINES AND MARINE MAMMAL REPRODUCTION [J].

ADDISON, RF .

CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES, 1989, 46 (02) :360-368

[2] CATALYTIC AND IMMUNOLOGICAL CHARACTERIZATION OF HEPATIC AND LUNG CYTOCHROMES P450 IN THE POLAR BEAR [J].

BANDIERA, SM ;

TOROK, SM ;

LIN, S ;

RAMSAY, MA ;

NORSTROM, RJ .

BIOCHEMICAL PHARMACOLOGY, 1995, 49 (08) :1135-1146

[3]

BARTOSEK I, 1979, J PHARMACOL METHOD, V3, P191

[4]

COOK D, 1993, TOXICOLOGIST, V13, P131

[5] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[6] Immunoquantitation and microsomal monooxygenase activities of hepatic cytochromes P4501A and p4502B and chlorinated hydrocarbon contaminant levels in polar bear (Ursus maritimus) [J].

Letcher, RJ ;

Norstrom, RJ ;

Lin, S ;

Ramsay, MA ;

Bandiera, SM .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1996, 137 (02) :127-140

[7]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[8] ORGANOCHLORINE CONTAMINANTS IN ARCTIC MARINE FOOD-CHAINS - IDENTIFICATION, GEOGRAPHICAL-DISTRIBUTION, AND TEMPORAL TRENDS IN POLAR BEARS [J].

NORSTROM, RJ ;

SIMON, M ;

MUIR, DCG ;

SCHWEINSBURG, RE .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1988, 22 (09) :1063-1071

[9]

OMURA T, 1964, J BIOL CHEM, V239, P2370

[10]

PUGNAGHI F, 1995, ISSX P, V8, P402

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