Evolution of supergene families associated with insecticide resistance

被引:485
作者
Ranson, H
Claudianos, C
Ortelli, F
Abgrall, C
Hemingway, J
Sharakhova, MV
Unger, MF
Collins, FH
Feyereisen, R
机构
[1] INRA, Ctr Rech Antibes, F-06560 Valbonne, France
[2] Univ Notre Dame, Ctr Trop Dis Res & Training, Notre Dame, IN 46556 USA
[3] Univ Liverpool, Liverpool Sch Trop Med, Liverpool L3 5QA, Merseyside, England
[4] Australian Natl Univ, Res Sch Biol Sci, Ctr Mol Genet Dev, Canberra, ACT 2601, Australia
[5] CSIRO Entomol, Canberra, ACT 2601, Australia
关键词
D O I
10.1126/science.1076781
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The emergence of insecticide resistance in the mosquito poses a serious threat to the efficacy of many malaria control programs. We have searched the Anopheles gambiae genome for members of the three major enzyme families-the carboxylesterases, glutathione transferases, and cytochrome P450s-that are primarily responsible for metabolic resistance to insecticides. A comparative genomic analysis with Drosophila melanogaster reveals that a considerable expansion of these supergene families has occurred in the mosquito. Low gene orthology and little chromosomal synteny paradoxically contrast the easily identified orthologous groups of genes presumably seeded by common ancestors. In A. gambiae, the independent expansion of paralogous genes is mainly a consequence of the formation of clusters among locally duplicated genes. These expansions may reflect the functional diversification of supergene families consistent with major differences in the life history and ecology of these organisms. These data provide a basis for identifying the resistance-associated enzymes within these families. This will enable the resistance status of mosquitoes, flies, and possibly other holometabolous insects to be monitored. The analyses also provide the means for identifying previously unknown molecules involved in fundamental biological processes such as development.
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页码:179 / 181
页数:3
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