Analysis of Fas and Fas ligand expression and function in lung cancer cell lines

The aim of this study was to investigate the expression of Fas and Fas ligand (FasL) and to determine the significance of these molecules in lung cancer cell lines. Immunoblotting, RT-PCR and flow cytometric analyses were carried out to measure the expression of Fas and Fast and to examine their interactions and effects on cell growth and apoptosis. Fas and Fast were coexpressed in most of the cell lines but to varying degrees. Apoptosis induced by the agonistic anti-Fas antibody was significantly correlated with Fas expression (P = 0.0075), whereas cisplatin-induced apoptosis was not. Upregulation of Fas and Fast expression by the administration of cisplatin was found in 7 of 11 (64%) and 9 of 11 (82%) cell lines, respectively. However, cisplatin-induced apoptosis was not suppressed by antagonistic anti-Fast antibody. Thus, our data indicated that Fas and Fast were co-expressed in lung cancer cell lines, and that Fas ligation induced by agonistic anti-Fas antibody is functional and induced apoptosis that was dependent on the levels of Fas expression. In contrast, Fas-FasL interactions appeared to be non-functional. Furthermore, our results suggest that cisplatin-induced apoptosis in lung cancer cells was independent of the Fas-Fast interaction. (C) 2000 Elsevier Science Ltd. All rights reserved.