Structural basis for ATP-dependent DnaA assembly and replication-origin remodeling

被引:236
作者
Erzberger, Jan P. [1 ]
Mott, Melissa L. [1 ]
Berger, James M. [1 ]
机构
[1] Univ Calif Berkeley, Div Biochem & Mol Biol, Dept Cell & Mol Biol, Berkeley, CA 94720 USA
关键词
D O I
10.1038/nsmb1115
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In bacteria, the initiation of replication is controlled by DnaA, a member of the ATPases associated with various cellular activities (AAA+) protein superfamily. ATP binding allows DnaA to transition from a monomeric state into a large oligomeric complex that remodels replication origins, triggers duplex melting and facilitates replisome assembly. The crystal structure of AMP-PCP-bound DnaA reveals a right-handed superhelix defined by specific protein-ATP interactions. The observed quaternary structure of DnaA, along with topology footprint assays, indicates that a right-handed DNA wrap is formed around the initiation nucleoprotein complex. This model clarifies how DnaA engages and unwinds bacterial origins and suggests that additional, regulatory AAA+ proteins engage DnaA at filament ends. Eukaryotic and archaeal initiators also have the structural elements that promote open-helix formation, indicating that a spiral, open-ring AAA+ assembly forms the core element of initiators in all domains of life.
引用
收藏
页码:676 / 683
页数:8
相关论文
共 51 条
[1]   Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex [J].
Bowman, GD ;
O'Donnell, M ;
Kuriyan, J .
NATURE, 2004, 429 (6993) :724-730
[2]   A MODEL FOR INITIATION AT ORIGINS OF DNA-REPLICATION [J].
BRAMHILL, D ;
KORNBERG, A .
CELL, 1988, 54 (07) :915-918
[3]  
Brunger AT, 1998, ACTA CRYSTALLOGR D, V54, P905, DOI 10.1107/s0907444998003254
[4]  
CLAREY, 2006, NAT STRUCT MOL 0709
[5]   Unraveling the early steps of prokaryotic replication [J].
Cunningham, EL ;
Berger, JM .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2005, 15 (01) :68-76
[6]   The DnaC helicase loader is a dual ATP/ADP switch protein [J].
Davey, MJ ;
Fang, LH ;
McInerney, P ;
Georgescu, RE ;
O'Donnell, M .
EMBO JOURNAL, 2002, 21 (12) :3148-3159
[7]   Evolutionary relationships and structural mechanisms of AAA plus proteins [J].
Erzberger, Jan P. ;
Berger, James M. .
ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2006, 35 :93-114
[8]   The structure of bacterial DnaA: implications for general mechanisms underlying DNA replication initiation [J].
Erzberger, JP ;
Pirruccello, MM ;
Berger, JM .
EMBO JOURNAL, 2002, 21 (18) :4763-4773
[9]   Replisome assembly at oriC, the replication origin of E-coli, reveals an explanation for initiation sites outside an origin [J].
Fang, LH ;
Davey, MJ ;
O'Donnell, M .
MOLECULAR CELL, 1999, 4 (04) :541-553
[10]   The Box VII motif of Escherichia coli DnaA protein is required for DnaA oligomerization at the E-coli replication origin [J].
Felczak, MM ;
Kaguni, JM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (49) :51156-51162