Isolation of microsatellite markers in mungbean, Vigna radiata

被引：45

作者：

Kumar, SV

Tan, SG ^{[1
]}

Quah, SC

Yusoff, K

机构：

[1] Univ Pertanian Malaysia, Dept Biol, Serdang 43400, Malaysia

[2] Univ Pertanian Malaysia, Dept Biochem & Microbiol, Serdang 43400, Malaysia

[3] Monash Univ Malaysia, Sch Sci & Engn, Bandar Sunway 46150, Petaling Jaya, Malaysia

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 02期

关键词：

microsatellites; mungbean; polymorphism; Vigna;

D O I：

10.1046/j.1471-8286.2002.00158.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid method for isolating microsatellite loci in mungbean, Vigna radiata, based on the 5'-anchored polymerase chain reaction technique revealed 23 microsatellite loci and six cryptically simple sequence repeats. We report on the characterization of seven polymorphic microsatellite loci in V. radiata. The number of alleles per locus ranged from 2 to 5 while the observed heterozygosity ranged from 0 to 0.9048. These markers should prove useful as tools for detecting genetic variation in mungbean varieties for germplasm management and crossbreeding purposes.

引用

页码：96 / 98

页数：3

共 6 条

[1]

Brachet S, 1999, MOL ECOL, V8, P160

[2] Single locus microsatellites isolated using 5' anchored PCR [J].