Properties of CD4+ T cells in human cytomegalovirus infection

被引:78
作者
Gamadia, LE
Rentenaar, RJ
van Lier, RAW
ten Berge, IJM
机构
[1] Univ Amsterdam, Acad Med Ctr, Expt Immunol Lab, NL-1105 AZ Amsterdam, Netherlands
[2] Univ Amsterdam, Acad Med Ctr, Dept Internal Med, Renal Transplant Unit, NL-1105 AZ Amsterdam, Netherlands
[3] Univ Amsterdam, Acad Med Ctr, Dept Clin Immunol & Rheumatol, NL-1105 AZ Amsterdam, Netherlands
[4] Univ Amsterdam, Acad Med Ctr, Dept Microbiol & Virol, NL-1105 AZ Amsterdam, Netherlands
关键词
cytomegalovirus; CD4(+) T cells; CD8(+) T cells; primary infection; latency;
D O I
10.1016/j.humimm.2004.02.020
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The correlates of protective immunity to disease-inducing viruses in man remain to be elucidated. We determined the kinetics and properties of cytomegalovirus (CMV)-specific CD4(+) T cells in healthy individuals and renal transplant recipients during different stages of CMV infection. Our data reveal that circulating CMV-specific CD4(+) T cells displayed an effector-memory phenotype, and produced the T helper 1 cytokines interferon-gamma and tumor necrosis factor-alpha. In addition, they lacked molecules for secondary lymphoid organ homing and expressed the cytotoxic molecule granzyme B, inferring a direct role of these cells at target sites of infection. In asymptomatic individuals the CMV-specific CD4(+) T-cell response preceded CMV-specific CD8(+) T-cell responses, whereas in symptomatic individuals the CMV-specific effector memory CD4(+) T-cell response was delayed and only detectable after antiviral therapy. The appearance of disease symptoms in these patients suggests that functional CD8(+) T cell and antibody responses are insufficient to control viral replication and that formation of effector memory CD4(+) T cells is necessary for recovery of infection. Human Immunology 65, 486-492 (2004). (C) American Society for Histocompatibility and Immunogenetics, 2004. Published by Elsevier Inc.
引用
收藏
页码:486 / 492
页数:7
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