Nitroazole universal bases in peptide nucleic acids

被引：20

作者：

Challa, H ^{[1
]}

Styers, ML ^{[1
]}

Woski, SA ^{[1
]}

机构：

[1] Univ Alabama, Dept Chem & Coalit Biomol Prod, Tuscaloosa, AL 35487 USA

来源：

ORGANIC LETTERS | 1999年 / 1卷 / 10期

关键词：

D O I：

10.1021/ol990270q

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHICS] The syntheses of RNA oligomers containing potential ambiguous nucleobase analogues, namely 3-nitropyrrole and 5-nitroindole, have been accomplished. Hybridization properties of these PNAs with complementary oligodeoxynucleotides were evaluated by thermal denaturation experiments. Both novel residues exhibited little variation in T-m(less than or equal to 1.5 degrees C) when positioned against any of the four nucleoside bases. The capability to incorporate degenerate sites should further expand the utility of PNA in applications where precise sequence information is not available.

引用

页码：1639 / 1641

页数：3

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